Mouse Model for Sjogren Syndrome (SS) Mus musculus (Mouse) Disease model

Sjögren's syndrome; SjS

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceInduced by submandibular gland
  • Model Animal StrainsBalb/c Mice(SPF), healthy, male, age: 4~6weeks, body weight:18g~20g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group(low,medium,high).
  • Modeling Period4-6 weeks
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  • Mouse Model for Sjogren Syndrome (SS) Packages (Simulation)
  • Mouse Model for Sjogren Syndrome (SS) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

Select the weight of 20 g, age of 8 weeks BALB/c mice, foot pad and back subcutaneous respectively in mice multi-point injection preparation in advance (in the same rat super clean bench, conventional surgical method to ta
ke out the submandibular gland, remove coated and connective tissue, with 0.5 g/L sodium azide saline cleaning the submandibular gland, with microscopic camber of crushed into the physiological saline, then in high speed mill, general submandibular gland homogenate, maintain 4 degrees, in ultrasonic crusher broken 1 h, put such as centrifuge (3 000 r/min, the centrifugal 10 mm), take the supernatant, determine the content of protein antigens. Antigen concentration dilution: the supernatant fluid with 0.1 mol/L PBS dilute liquid into 100 mg/L, the submandibular gland antigen and volume of the CFA blending, after the formation of water-in-oil solvent concentration of 200 mg/L) of the same rat submandibular gland tissues and cells mixed antigen (submandibular gland protein) 0.5 ml, pertussis vaccine in his back.Model mice were fed on no special pathogen under the condition of constant temperature, automatic water bottles of water.In the building schedule anesthesia to be put to death animals, removing submandibular gland tissue in 4% paraformaldehyde solution, fixed out after 6 h, add 20% sucrose solution at 4 degrees in the refrigerator overnight, the glands with frozen section machine cut into slices, HE dye, were observed under light.

Model evaluation

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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