Rabbit Model for Recurrent Aphthous Ulcer (RAC)
Recurrent oral ulce
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- FOB US$
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Overview
Properties
- Product No.DSI771Rb01
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Applicationsn/a
Research use only - Downloadn/a
- CategoryEye, ear, nose, throat and oral systems
- Prototype SpeciesHuman
- Sourceinduced by Recurrent
- Model Animal StrainsBig ears white rabbit, healthy, male and female, weight 1.5-2.5kg.
- Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
- Modeling Period4-6 weeks
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Modeling Method
"
Firstly, put the dialysis bag into the EDTA solution of 0.5mol / L for 0.5h and then solution freed, replace with distilled water and then cook 3 times, store in distilled water containing 0.01% sodium azide, store and reserve in 4 degrees. New Zealand rabbits weighted 1.5 ~ 2.0Kg were sacrificed by the anesthesia method. After homogenization of the oral mucosa, homogenate was prepared by homogenizer, ultrasonic oscillation, low temperature centrifugation, retention of sediment. The supernatant was collected and dialyzed To filter small molecules, the macromolecules remain in the dialysis bag. Dialysis process repeate 5 times, and finally the contents of the bag freeze-dried to obtain powdered protein as antigen, placed in -70 degrees low temperature refrigerator spare. New Zealand rabbits weighted 1.5 to 2.0 kg, 12.5 mg of pre-extracted oral mucosal protein dissolved in 0.5 ml of phosphate buffer, add 5 ml of complete Freund's adjuvant to the emulsion. Inject the above tissue emulsion 1 ml into rabbit spine on both sides of the skin, 20 times in total. Inject 1 time every 2 weeks in the same way, 5 times in total. Cut oral mucosa for routine pathological examination at different times."
Model evaluation
Pathological results
Cytokines level
Statistical analysis
SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±s), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.
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- Tissue/Sections Customized Service
- Serums Customized Service
- Immunohistochemistry (IHC) Experiment Service
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