Rat Model for Acute Gastric Ulcer (AGU)

1. Modeling: SD rats were randomly divided into sham operation group, model control group, model test group, model positive group. Rats in the model group were treated with intraperitoneal injection of pentobarbital sodium and fixed on the operation table. The abdominal operation was treated with iodophor in the abdominal operation. After the incision of the abdominal xiphoid was exposed to the stomach, the transfer of the pylorus and duodenum At the end of the line will be pylorus stitches after suture abdominal wall incision. Sham operation tissue open line is not tied, other operations are the same.
2. Administration and post-treatment: Rats in each group were given intravenous injection of the corresponding drug solution immediately after the model, and the model group and normal control group were given equal volume of normal saline. After administration, the animals were placed in the cage and the rats were sacrificed for 18 hours after fasting for 14 days. The pH value of gastric juice, the activity of H +, K + -ATPase and the activity of pepsin were measured.
3. Specimen collection: After the model, the stomach of the rats was collected and the gastric juice was collected in a centrifuge tube. Centrifuge at 3000 r / min for 10 min to obtain supernatant in the storage tube for the subsequent gastric pH and pepsin activity Determination. The rats' stomach was washed with ice saline and used for the detection of H +, K + -ATPase activity in gastric mucosa.

1. PH value of gastric juice: Determination of pH value of gastric juice
2. Detection of pepsin activity: pepsin activity was determined by the method of pepsin assay kit.
3. Detection of H+ and K+-ATP activity in gastric mucosa: the activity of H+ and K+-ATP in gastric mucosa of rats were measured by the supernatant samples of 5% gastric mucosa homogenate.

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.