Rat Model for Osteoporosis (OP)

1. Weigh the rats and perform intraperitoneal injection to anesthetize them. After shaving in the middle of the abdomen, wipe the surgical area with iodine and alcohol.
2. Cut open the abdominal cavity along the white line of the abdomen, separate the uterus, and remove the ovaries. After cleaning the wound, sew the skin and base layer in two layers. After the rat wakes up, put it back into a clean cage and return it to the breeding room for feeding. Observe the state and death of the rats and keep records regularly .
3. Rats are injected with 400000 units of penicillin daily to prevent infection three days after surgery,
4. The control group only removed about 1g of fat around the ovaries, while retaining the ovaries. The other treatments are the same.
5. After 8 weeks of surgery, the specimens are executed and collected.

1. The serum testosterone level of the model group rats is significantly decreased, while the estradiol levels is increased. The biochemical examination results shows that the bone formation indicators of the model group: the serum alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRAP) activities of the rats are significantly reduced; The24-hour urine hydroxyproline concentration, creatinine, calcium, and creatinine levels significantly are increased.

Take tibia specimens from each group of rats, fix them for 24-48 hours, and then undergo decalcification. Replace the decalcification solution every 5-7 days; After complete decalcification, gradient ethanol dehydration, xylene transparency, longitudinal paraffin embedding, 5um sectioning, routine HE staining. The tibia of control group rats' trabeculae are dense and interwoven into a network; The number of trabeculae in the model group are significantly decreased, and the trabeculae became thinner and fractured.

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.