Rat Model for Rickets Rattus norvegicus (Rat) Disease model

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceInduced by Vitamin D deficient diet and avoid light feeding
  • Model Animal StrainsSD young rats (SPF), 21 days age, half male and half female
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group
  • Modeling Period6~8 weeks
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  • Rat Model for Rickets Packages (Simulation)
  • Rat Model for Rickets Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

1. Rickets group was fed with vitamin D and pure water, while the control group was fed with basic feed and tap water. Rats were fed for 6 weeks, and the bone mineral density (BMD) was measured by absortionmetry and DEXA (dual-energy X-ray).
2. Feed formula (%): corn flour 30g, bran 35g, fish 8g, soybean 20g, yeast 5g, 1g powder, salt 0.5g, methionine 0.1g, VitD 3 0.1g, a variety of vitamin 0.2g; the content of calcium was 1.18%. High calcium feed formula: on the basis of basic feed, coupled with calcium lactate and the right amount of bone meal to make up 1.8% of the total calcium. Lack of VitD feed formula (%): corn flour 76g, egg white powder 5g, wheat gluten 13g, calcium carbonate 5g, sodium chloride 1g (the formula by the Yunnan provincial science and Technology Commission does not contain VitD).
3. Animals were placed in a light room, and the rats were fed in a cage. Weight and body length were measured once a week.

Model evaluation

Results:
1. The epiphyseal end of the rats in the rickets group was enlarged, and the appearance of the brush and cup mouth changed.
2. The length and weight of rickets rats were significantly different from those of the control group. The lumbar spine BMD of rickets rats was lower than that of normal control group.
3. Lumbar spine bone mineral density of rickets rats compared with the normal control group, the BMD of lumbar spine was significantly lower than that of normal control group in BMD.

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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