CLIA Kit for Calcitonin Gene Related Peptide (CGRP) Rattus norvegicus (Rat) Competition CLIA

CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta

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  • CLIA Kit for Calcitonin Gene Related Peptide (CGRP) Packages (Simulation)
  • CLIA Kit for Calcitonin Gene Related Peptide (CGRP) Packages (Simulation)
  • CLIA Kit for Calcitonin Gene Related Peptide (CGRP) Results demonstration
  • CCA876Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Calcitonin Gene Related Peptide (CGRP) and the recovery rates were calculated by comparing the measured value to the expected amount of Calcitonin Gene Related Peptide (CGRP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-104 96
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 88-102 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin Gene Related Peptide (CGRP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcitonin Gene Related Peptide (CGRP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-98% 99-105% 78-99% 90-102%
EDTA plasma(n=5) 94-101% 79-103% 79-90% 79-94%
heparin plasma(n=5) 83-91% 80-88% 81-95% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

CLIA Kit for Calcitonin Gene Related Peptide (CGRP)

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Calcitonin Gene Related Peptide (CGRP). A competitive inhibition reaction is launched between biotin labeled Calcitonin Gene Related Peptide (CGRP) and unlabeled Calcitonin Gene Related Peptide (CGRP) (Standards or samples) with the pre-coated antibody specific to Calcitonin Gene Related Peptide (CGRP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Calcitonin Gene Related Peptide (CGRP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Calcitonin Gene Related Peptide (CGRP) level in the sample or standard.

Citations

  • Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo.Hindawi: 190370
  • Increased levels of CGRP in peripheral blood in women with chronic migraine: A reliable biological markerPubMed: PMC3620076
  • CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic MigraineOnlinelibrary: head.12372
  • Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid APubmed:Pmc4028234
  • Prognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot.Pubmed:24624298
  • Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control studyPubmed:24943060
  • OnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migrainePubMed: 25735000
  • Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in ratsPubMed: 25892548
  • Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitisPubmed:27156748
  • Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patientsPubmed:27250233
  • Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction.Pubmed:27144592
  • Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кBPubmed:27150105
  • Prevention of Bone Loss in a Model of Postmenopausal Osteoporosis throughAdrenomedullin Inhibition.pubmed:27445864
  • Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis.pubmed:27896372
  • A new comorbidity model and the common pathological mechanisms of migraine and epilepsypubmed:28559979
  • Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndromepubmed:28483500
  • CNS myeloid cells critically regulate heat hyperalgesiaPubmed:29634489
  • Feasibility of serum CGRP measurement as a biomarker of chronic migraine: a critical reappraisalPubmed:30006780
  • RAMP1 in Kupffer cells is a critical regulator in immune-mediated hepatitis10.1101:357582
  • MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL
  • Periodontal inflammation is related to increased serum calcitonin gene‐related peptide (CGRP) levels in patients with chronic migrainePubmed: 31070784
  • Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signalingPubmed: 31420791
  • Electrical stimulation of the superior sagittal sinus suppresses A-type K+ currents and increases P/Q-and T-type Ca 2+ currents in rat trigeminal ganglion …
  • Effects of nanoparticles on Neuroinflammation in a Mouse Model of AsthmaPubmed: 31542455
  • Electrical stimulation of the superior sagittal sinus suppresses A-type K currents and increases P/Q-and T-type Ca 2 currents in rat trigeminal ganglion …Pubmed: 31375062
  • Effects of endurance exercise on serum concentration of calcitonin gene-related peptide (CGRP): a potential link between exercise intensity and headachePubmed: 32286239
  • Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practicePubmed: 32951099
  • Mild systemic inflammation enhances response to OnabotulinumtoxinA in chronic migraineurs33441852
  • Perirenal adipose afferent nerves sustain pathological high blood pressure in ratsPubmed:35668093

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