On May 23,2022, professor Yiguang Wang from State Key Laboratory of Natural and Biomimetic Drugs, School of PharmaceuticalSciences,Peking University published a paper titled A pyroptosis nanotumer for cancer therapy on Nature Namotechmology.Pyroptosis is a gasdermin-mediated programmed necrosis that occurs via membrane perforation and that can be exploited for biomed-ical applications in cancer therapy. However, inducing specific pyroptotic cancer cell death while sparing normal cells is challenging.Here, we report an acid-activatable nanophotosensitizer library that can be used to spatiotemporally target distinctstages of endosomalmaturation, cnabling tumnable cellular pyroptosis. Spe......

Central nervous system(CNS)injury is one of the leading causes of death and disability.Although the causes of injury are diverse, the common end result is substantial neuronal damage, or neuronal loss, in the affected region. This is thought to underlie the cognitive, sensorimotor function and personality changes typically seen in patients. To date, drug treatments adopting a ‘neuro-centric’ approach have failed to deliver notable clinical benefits for the treatment of CNS injury. Recently, a number of literatures have reported potential therapeutic methods for brain injury, which may help improve the prognosis of patients with brain injury.1. Astrocyte-targeted gene delivery of IL-2 s......

On May 6, 2022, Ching-Hsiu Tsai, Graduate Institute of Biotechnology, National Chung Hsing University, Taiwan, and his team published a paper titled “The gibberellic acid derived from the plastidial MEP pathway is involved in the accumulation of Bamboo mosaic virus” in New Phytologist, which confirmed the de novo synthesis of gibberellic acid could assist BaMV accumulation.The kits [ELISA Kit for Gibberellic Acid (GA), CEA759Ge] of Cloud-Clone brand was chosed in this article, we are so proud for supporting the reaserchers.A gene upregulated in Nicotiana benthamiana after Bamboo mosaic virus (BaMV) infection was revealed as 1-deoxy-D-xylulose-5-phosphate reductoisomerase (NbDXR). DXR i......

Cardiovascular diseases (CVD), including atherosclerosis, hypertension, heart failure, atrial fibrillation and myocardial fibrosis, are associated with high morbidity and mortality. Many risk factors are widely known, such as smoking, poor dietary habits, obesity, diabetes mellitus and high cholesterol, but these cannot explain all CVD incidences. Accumulating evidence has demonstrated that gut microbiome and their metabolites play a pivotal role in the onset and progression of CVD. Recently, several studies have reported the correlation between intestinal microbes and CVD, which may provide help for the prevention and treatment of CVD.1. Interplay between diet and gut microbiome, and ......

Previously we introducedthe common samples preparation methods including the preparation of blood(serum, plasma), tissue homogenates, cell lysates,etc.Nowwe’ll present preparation methods of uncommonsamples,such as sputum,bronchoalveolar lavage fluid, saliva, milk, hydrothorax and ascite, semen, etc.1.SputumWeightthe viscous part of sputum,mix the sputum with 0.1 % DTT (dithiothreitol) (two fold volume) and shake for 5 minutes at 37°C.Add PBS (twofold volume) andshake again for another 15-20 minutes. After Filtering through a 150 mesh wire net, remove particulates by centrifugation ofapproximately 1,000×gfor 10 minutes.Collect the supernatant and assay immediately or store samples in a......

On 17 May 2022,Martin Schepelmann, School of Biosciences, Cardiff University, United Kingdom, and his team published a paper titled “Impaired Mineral Ion Metabolism in a Mouse Model of Targeted Calcium-Sensing Receptor (CaSR) Deletion from Vascular Smooth Muscle Cells” in Journal of the American Society of Nephrology, which suggested that the VSMC-CaSR directly contributes to total body mineral ion homeostasis.The kits [ELISA Kit for Klotho (KL), SEH757Mu] of Cloud-Clone brand was chosed in this article, we are so proud for supporting the reaserchers.Background: Impaired mineral ion metabolism is a hallmark of chronic kidney disease (CKD) -metabolic bone disorder. It can lead to pathol......