1. Baking:Bake the IHC slides for 0.5~2 hours at60℃.2. Deparaffinization and rehydration: After thebaking, put slides into the following reagents in order:1)XyleneI: 30 minutes2)XyleneII: 30 minutes3)100%ethanol: 10 minutes4)95%ethanol: 10 minutes5)80%ethanol: 10 minutes6)70%ethanol: 10 minutes7)Rinse sections with running water 10 minutes3. Antigen retrieval - Microwave heat antigenretrieval (optional): Transfer the slides into a boiling EDTA-TRIS solution,and ensure the slides are soaked completely. Low fire for one minute, stopheating and keep in microwave for 10min, then another low fire for 3-4 min, andallow slides to cool in the EDTA-TRIS solution at room temperature.4.Rinse 3X i......

From the discovery of the structure of DNA makes people have a preliminary understanding of gene structure, to the emergence of PCR technology to achieve gene amplification in vitro, and then to the PCR based gene sequencing technology. The first time we have seen the whole information of human genome library. But no matter how the genetic manipulation technique develops, it can not change people's fanatical pursuit of gene editing technology, and that because it’s directly related to human diseases of diagnosis and treatment, bear and rear better children and the evolution of race. Gene editing technology has gone through 3 stages of development. The first and second generation te......

Primary liver cancer is one of the most common malignant tumors, according to statistics, the annual mortality of liver cancer is second highest of all cancers’ mortality, morbidity and mortality of liver cancer in China is the largest in the world, accounting for 55% of the new incidence and deaths each year. And the number of liver cancer patients is increasing year by year. Because of its high malignant degree, aggressiveness, rapid growth, easy recurrence, metastasis and other biological characteristics, it is known as the 'king of cancer'. Despite the current various surgical, chemotherapy and other treatment methods continue to improve, the effect is still poor. In recent......

The relationship between microbes and human health has always been concerned. In recent years, the study of gut microbiota has become the current hotspot in life sciences and medical research with the application of high-throughput sequencing and metagenomics, more and more scientific researchers have forced on this field. Scientists find that gut microbiota can participate in and regulate the body's physiological functions, affect weight and digestion, defense a variety of diseases caused by the exogenous infections. In addition, it is closely related with some endogenous diseases, like endocrine disease, intestinal disease, neurological disease, cancer and so on. One article, nam......

Pleural mesothelioma is the tumor that originated in the peritoneal mesothelial cell. Pleural mesothelioma is related with asbestos exposure. There are significant differences in the incidence of pleural mesothelioma in different countries, this is mainly related to the asbestos consumption of these countries over the past several decades. The mortality rate of pleural mesothelioma has a rising tendency in recent years.The common first symptom of pleural mesothelioma is chest pain, cough and shortness of breath. There are also symptoms of fever, sweating or joint pain. Most patients have a lot of pleural effusion and short of breath, the patients that without mass of pleural effusion w......

‍‍‍Since September 2004, "Nature" published the first article on the results of RNA interference by American scientist Andrew Z. Fire and Craig C. Mello et al., The small RNA playsthe important regulatory role in biological life activities. Every day there are articles on relation of the various small RNA and body development. And smart biotechnology companies are also at an amazing rate to actively use RNAi technology fordrug screening,Cloud-Clone Corp. has been launched for the lentivirus and adenovirus expression system, The RNAi is one of the important sever of customer.RNAi is a specific gene silencing technology,which is mediated by double-stranded RNA (dsRNA). RNAi bl......