CLIA Kit for Defensin Beta 1 (DEFb1)
B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1
- UOM
- FOB US$ 559.00 US$ 798.00 US$ 3,591.00 US$ 6,783.00 US$ 55,860.00
- Quantity
Overview
Properties
- Product No.SCB373Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsChemiluminescent immunoassay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunityHepatology
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Recovery
Matrices listed below were spiked with certain level of recombinant Defensin Beta 1 (DEFb1) and the recovery rates were calculated by comparing the measured value to the expected amount of Defensin Beta 1 (DEFb1) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 94-105 | 101 |
EDTA plasma(n=5) | 87-95 | 90 |
heparin plasma(n=5) | 95-104 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Beta 1 (DEFb1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Beta 1 (DEFb1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Defensin Beta 1 (DEFb1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 83-91% | 80-88% | 81-95% | 98-105% |
EDTA plasma(n=5) | 80-101% | 95-104% | 96-105% | 87-96% |
heparin plasma(n=5) | 92-99% | 82-101% | 96-103% | 80-91% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Substrate A | 1×10mL | Substrate B | 1×2mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle
The microplate provided in this kit has been pre-coated with an antibody specific to Defensin Beta 1 (DEFb1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Defensin Beta 1 (DEFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Defensin Beta 1 (DEFb1) level in the sample or standard.;
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Citations
- Modulation of Porcine β-Defensins 1 and 2 upon Individual and Combined Fusarium Toxin Exposure in a Swine Jejunal Epithelial Cell LineAsm: Source
- Identification of sociodemographic and clinical factors associated with the levels of human β-defensin-1 and human β-defensin-2 in the human milk of Han ChinesePubmed: 24124699
- Concentration characteristics of bovine β-defensin 1 and 2 in fresh bovine milk and infant formulaDoi: 10.1111
- Green tea polyphenol epigallocatechin-3-gallate improves epithelial barrier function by inducing the productionof antimicrobial peptide pBD-1 and pBD-2 in monolayers of porcine intestinal epithelial IPEC-J2 cells.Pubmed:26991948
- Swine-Derived Probiotic Lactobacillus plantarum Inhibits Growth and Adhesion of Enterotoxigenic Escherichia coli and Mediates Host DefensePubmed:29997590
- Caprylic acid and nonanoic acid upregulate endogenous host defense peptides to enhance intestinal epithelial immunological barrier function via histone deacetylase …Pubmed: 30342347
- EFFECT OF NIOSOMAL ANTIMICROBIAL PEPTIDE HBD-1 ON THE HEALING RATE OF INFECTED WOUNDS IN RATS
- Effect of antimicrobial peptides HNP‐1 and hBD‐1 on Staphylococcus aureus strains in vitro and in vivoPubmed: 31313350
- Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitisPubmed: 31927460
- Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport33375218
- Human beta defensin levels and vaginal microbiome composition in post-menopausal women diagnosed with lichen sclerosus34362937
- Isolation, culture, and identification of ceruminous gland cellsPubmed:35113280