CLIA Kit for Estrogen Receptor Beta (ERb) Homo sapiens (Human) Sandwich CLIA

ER-B; ESRB; ESTRB; NR3A2; NR3-A2; ESR2; Estrogen Receptor 2; Nuclear Receptor Subfamily 3,Group A,Member 2

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • CLIA Kit for Estrogen Receptor Beta (ERb) Packages (Simulation)
  • CLIA Kit for Estrogen Receptor Beta (ERb) Packages (Simulation)
  • CLIA Kit for Estrogen Receptor Beta (ERb) Results demonstration
  • SCA437Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Estrogen Receptor Beta (ERb) and the recovery rates were calculated by comparing the measured value to the expected amount of Estrogen Receptor Beta (ERb) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-105 101
EDTA plasma(n=5) 85-99 93
heparin plasma(n=5) 80-95 82

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Estrogen Receptor Beta (ERb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Estrogen Receptor Beta (ERb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 91-99% 90-98% 88-101%
EDTA plasma(n=5) 86-95% 78-99% 86-101% 84-98%
heparin plasma(n=5) 83-97% 98-105% 80-95% 87-94%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Estrogen Receptor Beta (ERb)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Estrogen Receptor Beta (ERb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Estrogen Receptor Beta (ERb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Estrogen Receptor Beta (ERb) level in the sample or standard.;

Citations

  • Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signallingPubmed:24846829
  • Differential expression of estrogen receptor α and β isoforms in multiple and solitary leiomyomasPubMed: 26529545
  • Selective Aryl Hydrocarbon Receptor Modulator 3,3′-Diindolylmethane Impairs AhR and ARNT Signaling and Protects Mouse Neuronal Cells Against HypoxiaPubMed: 26476840
  • Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR31 signallingPubMed: 24846830
  • Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblastsscience:S1001074216300924
  • Involvement of BDNF/TrkB and ERK/CREB axes in nitroglycerin-induced rat migraine and effects of estrogen on these signals in the migraine.pubmed:27875242
  • Postmenopausal osteoporosis is associated with the regulation of SP, CGRP, VIP, and NPYPubmed:29807224
  • Physical Signs of Illness, Liver Functions and Reproductive Parameters of Female Rats Supplemented with Melaleuca cajuputi Methanolic ExtractPbulication:325682424
  • In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts
  • MiR-145 regulates steroidogenesis in mouse primary granulosa cells through targeting Crkl34273377
  • Modifying effect of obesity on the content of sex hormones and their receptors in endometrial adenocarcinoma and its surrounding tissue

Recommend products