CLIA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF) 
ATF1; ATF2; HFB1-GDNF; Astrocyte-Derived Trophic Factor; Glial Derived Neurotrophic Factor
- UOM
- FOB US$ 588.00 US$ 840.00 US$ 3,780.00 US$ 7,140.00 US$ 58,800.00
- Quantity
Overview
Properties
- Product No.SCA043Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsChemiluminescent immunoassay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCytokineApoptosisNeuro science
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant Glial Cell Line Derived Neurotrophic Factor (GDNF) and the recovery rates were calculated by comparing the measured value to the expected amount of Glial Cell Line Derived Neurotrophic Factor (GDNF) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 93-101 | 96 |
| EDTA plasma(n=5) | 87-96 | 90 |
| heparin plasma(n=5) | 80-92 | 81 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glial Cell Line Derived Neurotrophic Factor (GDNF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glial Cell Line Derived Neurotrophic Factor (GDNF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 93-101% | 84-94% | 99-105% | 98-105% |
| EDTA plasma(n=5) | 99-105% | 81-98% | 78-91% | 86-95% |
| heparin plasma(n=5) | 89-97% | 92-99% | 80-101% | 83-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Substrate A | 1×10mL | Substrate B | 1×2mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Test principle
The microplate provided in this kit has been pre-coated with an antibody specific to Glial Cell Line Derived Neurotrophic Factor (GDNF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Glial Cell Line Derived Neurotrophic Factor (GDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glial Cell Line Derived Neurotrophic Factor (GDNF) level in the sample or standard.;
Giveaways
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CLIA Kit Customized Service
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Streptavidin-Agarose Beads
Citations
- Efficient Generation of Functionally Active Spinal Cord Neurons from Spermatogonial Stem Cellspubmed:27566610
- Safflower Extract and Aceglutamide Injection Promoting Recovery of Peripheral Innervations via Vascular Endothelial Growth Factor-B Signaling in Diabetic MicePMC5717862
- Acupuncture Alters Pro-infl ammatory Cytokines in the Plasma of Maternally Separated Rat Pupspubmed:28986807
- GDNF serum levels are found to be lower in opioid-maintained patientsISSN 2531-4122
- Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in RatsPubmed: 30974849
- The Effects of Nordic Walking With Poles With an Integrated Resistance Shock Absorber on Cognitive Abilities and Cardiopulmonary Efficiency in …Pubmed: 33192480
- Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡33389158
- The effect of biodegradable silk fibroin-based scaffolds containing glial cell line-derived neurotrophic factor (GDNF) on the corneal regeneration process34119551
- Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathwayPubmed:35366745
- Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377