CLIA Kit for Glycated Hemoglobin A1c (HbA1c) Homo sapiens (Human) Competition CLIA

Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

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  • CLIA Kit for Glycated Hemoglobin A1c (HbA1c) Packages (Simulation)
  • CLIA Kit for Glycated Hemoglobin A1c (HbA1c) Packages (Simulation)
  • CLIA Kit for Glycated Hemoglobin A1c (HbA1c) Results demonstration
  • CCA190Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Glycated Hemoglobin A1c (HbA1c) and the recovery rates were calculated by comparing the measured value to the expected amount of Glycated Hemoglobin A1c (HbA1c) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-93 81
EDTA plasma(n=5) 83-93 88
heparin plasma(n=5) 83-91 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycated Hemoglobin A1c (HbA1c) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycated Hemoglobin A1c (HbA1c) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glycated Hemoglobin A1c (HbA1c) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-91% 85-93% 88-102% 81-103%
EDTA plasma(n=5) 82-94% 78-101% 84-97% 79-99%
heparin plasma(n=5) 80-90% 82-93% 91-105% 78-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

CLIA Kit for Glycated Hemoglobin A1c (HbA1c)

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Glycated Hemoglobin A1c (HbA1c). A competitive inhibition reaction is launched between biotin labeled Glycated Hemoglobin A1c (HbA1c) and unlabeled Glycated Hemoglobin A1c (HbA1c) (Standards or samples) with the pre-coated antibody specific to Glycated Hemoglobin A1c (HbA1c). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glycated Hemoglobin A1c (HbA1c) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Glycated Hemoglobin A1c (HbA1c) level in the sample or standard.

Citations

  • The Effect of Vitamin D Supplementation on Glycemic Control and Lipid Profile in Patients with Type 2 Diabetes MellitusPubMed: 26391639
  • The Effect of Vitamin D Supplementation on Glycemic Control and Lipid Profile in Patients with Type 2 Diabetes Mellitus.pubmed:26391639
  • Circulating Betatrophin and Hepatocyte Growth Factor in Type 2 Diabetic Patients: Their Relationship With Disease Prognosis405
  • Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitropubmed:28105758
  • d-Ribose as a Contributor to Glycated Haemoglobinpubmed:29033370
  • Hypoglycemic effect of Hypericum attenuatum Choisy extracts on type 2 diabetes by regulating glucolipid metabolism and modulating gut microbiota
  • Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.
  • Stressors, allostatic load, and health outcomes among women hotel housekeepers: A pilot studyPubmed: 30615593
  • Parabacteroides distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile AcidsPubmed: 30605678
  • Gymnemic acid alleviates type 2 diabetes mellitus and suppresses endoplasmic reticulum stress in vivo and in vitro
  • 1H Nuclear Magnetic Resonance (NMR)-Based Cerebrospinal Fluid and Plasma Metabolomic Analysis in Type 2 Diabetic Patients and Risk Prediction for Diabetic …Pubmed: 31248127
  • Gymnemic Acid Alleviates Inflammation and Insulin Resistance via PPARδ-and NFκB-mediated pathways in db/db mice.
  • Duodenal-Jejunal Bypass Ameliorates Type 2 Diabetes Mellitus by Activating Insulin Signaling and Improving Glucose Utilization in the BrainPubmed: 31605365
  • Modulation of gut microbiota contributes to effects of intensive insulin therapy on intestinal morphological alteration in high-fat-diet-treated micePubmed: 31749050
  • Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic RetinopathyPubmed: 32165929
  • Activation of a Specific Gut Bacteroides-Folate-Liver Axis Benefits for the Alleviation of Nonalcoholic Hepatic SteatosisPubmed: 32783933
  • The effects of different intensity endurance and resistance exercise on diabetic-related blood profiles in impaired glucose tolerance mice
  • Relation of pharmacopenile duplex ultrasonography parameters and glycated hemoglobin (HbA1c) in diabetic patients with erectile dysfunction33334711
  • Identification of Key Contributive Compounds in a Herbal Medicine: A Novel Mathematic¡ªBiological Evaluation Approach
  • The Roles of Liver Inflammation and the Insulin Signaling Pathway in PM2. 5 Instillation-Induced Insulin Resistance in Wistar Rats34745386
  • Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced MicePubmed:35740008
  • P001–Inv. Clínica

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