CLIA Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Homo sapiens (Human) Sandwich CLIA

G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH

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  • CLIA Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Packages (Simulation)
  • CLIA Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Packages (Simulation)
  • CLIA Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Results demonstration
  • SCB932Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) level in the sample or standard.;

Citations

  • Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid LeukemiaPubMed: 26408402
  • Inactivation of the p53–KLF10–CEBPA Axis in Acute Myeloid LeukemiaPubMed: 26408402
  • Dapagliflozin modulates glucagon secretion in an SGLT2-independent manner in murine alpha cells.pubmed:28499695
  • Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egressionpubmed:28720868
  • Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipidsPubmed: 30237167
  • Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogelsPubmed: 30343256
  • Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood CellsPubmed: 30488202
  • Discovery, Optimization and Target Identification of Novel Potent Broad-Spectrum Antiviral InhibitorsPubmed: 30938999
  • Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathwayPubmed: 31078923
  • Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid LeukemiaPubmed: 31718075
  • Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor …Pubmed: 32046779
  • Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathwayPubmed: 32028241
  • Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in ratsPubmed: 33170419
  • Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo
  • Regulation of hepatic lipid metabolism in CB1-/-and Abcb4-/--HBV surface protein transgenic mice
  • BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 58133540760
  • Omega-3 Eicosapentaenoic Acid Reduces Prostate Tumor Vascularity33262291
  • Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026
  • Curcumin alters distinct molecular pathways in breast cancer subtypes revealed by integrated miRNA/mRNA expression analysisPubmed:34981672
  • Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegenerationPubmed:35151792
  • Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiencyPubmed:35577991

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