CLIA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Mus musculus (Mouse) Sandwich CLIA

HB-EGF; DTR; DTS; DTSF; HEGFL; Diphtheria Toxin Receptor; Proheparin-binding EGF-like growth factor; Diphtheria toxin receptor

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  • CLIA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Packages (Simulation)
  • CLIA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Packages (Simulation)
  • CLIA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Results demonstration
  • SCB479Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) and the recovery rates were calculated by comparing the measured value to the expected amount of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-89 85
EDTA plasma(n=5) 86-98 95
heparin plasma(n=5) 84-102 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 82-103% 82-97% 89-98%
EDTA plasma(n=5) 88-102% 86-102% 99-105% 91-98%
heparin plasma(n=5) 90-97% 84-94% 78-91% 86-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) level in the sample or standard.;

Citations

  • Astrocyte ERK phosphorylation precedes K+-induced swelling but follows hypotonicity-induced swelling.PubMed: 21118399
  • Cypermethrin Induces Astrocyte Apoptosis by the Disruption of the Autocrine/Paracrine Mode of Epidermal Growth Factor Receptor SignalingPubmed: source
  • 軽度炎症性膀胱痛マウスモデルの構築とNGFの関与Jstage:Source
  • A novel method for assessing bladder-related pain reveals the involvement of nerve growth factor in painassociated with cyclophosphamide-induced chronic cystitis in mice.Pubmed:25820250
  • 2 型糖尿病モデルラットにおける糖尿病性腎症の進行とHB-EGF の関連性17844
  • Heparin-binding epidermal growth factor contributes to COPD disease severity by modulating airway fibrosis and pulmonary epithelial–mesenchymal transitionPubmed:29581578
  • CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17
  • Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growthPubmed: 30769285
  • Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheetsPubmed: 33164313
  • 象牙芽細胞の枯渇は象牙芽細胞分化と象牙質形成を誘導する

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