CLIA Kit for Hepcidin (Hepc) Homo sapiens (Human) Sandwich CLIA

HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • CLIA Kit for Hepcidin (Hepc) Packages (Simulation)
  • CLIA Kit for Hepcidin (Hepc) Packages (Simulation)
  • CLIA Kit for Hepcidin (Hepc) Results demonstration
  • SCB979Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Hepcidin (Hepc) and the recovery rates were calculated by comparing the measured value to the expected amount of Hepcidin (Hepc) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-99 93
EDTA plasma(n=5) 78-101 91
heparin plasma(n=5) 82-102 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepcidin (Hepc) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepcidin (Hepc) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hepcidin (Hepc) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-103% 80-103% 79-102% 85-93%
EDTA plasma(n=5) 87-95% 83-104% 86-99% 92-104%
heparin plasma(n=5) 88-102% 78-95% 97-104% 82-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Hepcidin (Hepc)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Hepcidin (Hepc). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepcidin (Hepc). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepcidin (Hepc) level in the sample or standard.;

Citations

  • Hepcidin expression in colon during trinitrobenzene sulfonic acid-induced colitis in ratsPubmed: 24764672
  • Modulations of serum hepcidin, IL6 and iron status by different exercise regimens in ovarectomized ratsCom:Source
  • Serum iron metabolism markers including hepcidin in multiple sclerosis patientsSpringer:Source
  • Monitoring Hepcidin Level in Chronic Hepatitis C Virus Patients during TherapyZu:Source
  • Gluconeogenic signals regulate iron homeostasis via hepcidin in micePubmed:Pmc3989026
  • Inappropriate expression of hepcidin by liver congestion contributes to anemia and relative iron deficiencyPubmed:24440572
  • The action of JAK, SMAD and ERK signal pathways on hepcidin suppression by polysaccharides from Angelica sinensis in rats with iron deficiency anemiaPubmed:24752529
  • Changes in skeletal muscle iron metabolism outpace amyotrophic lateral sclerosis onset in transgenic rats bearing the G93A hmSOD1 gene mutationPubmed:25175826
  • Hepcidin and regulation of iron homeostasis in maintenance hemodialysis patientsPubmed:25193892
  • The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disordersPubmed:25245337
  • An Evaluation of the Correlation between Hepcidin Serum Levels and Disease Activity in Inflammatory Bowel DiseasePubmed:25628652
  • Serum hepcidin levels in Bulgarian population.Pubmed:25651734
  • Liver Congestion in Heart Failure Contributes to Inappropriately Increased Serum Hepcidin despite AnemiaPubmed:25742771
  • Hepcidin regulation in prostate and its disruption in prostate cancerPubMed: 25858146
  • B-cell translocation gene 2 promotes hepatic hepcidin production via induction of Yin Yang 1PubMed: 25839654
  • Effect of a More Permeable Dialysis Membrane on ESA Resistance in Hemodialysis Patients - A Pilot InvestigationPubMed: 26536083
  • Urinary Hepcidin in Diagnosis of Iron Deficiency AnemiaOpenview: E01Ad7C4A9109Ed5Fadb03Ec206D7Bdc
  • Ferrous sulfate, but not iron polymaltose complex, aggravates local and systemic inflammation and oxidative stress in dextran sodium sulfate-induced colitis in ratsPubMed: 25852597
  • Iron Supplementation Attenuates the Inflammatory Status of Anemic Piglets by Regulating HepcidinPubMed: 25774043
  • Kupffer cell depletion protects against the steatosis, but not the liver damage, induced by marginal copper, high fructose diet in male rats.PubMed: 25813056
  • Hepcidin in tumor‐related iron deficiency anemia and tumor‐related anemia of chronic disease: pathogenic mechanisms and diagnosisPubMed: 24954786
  • The Effects of Treatment on Serum Hepcidin and Iron Homeostasis in HIV-1-Infected In-dividualsJournal: 59107
  • Hepcidin and iron metabolism associated with cardiometabolic risk factors in children: A case-control study.Pubmed:27139516
  • The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing micePubmed:26757699
  • Hydrogen sulfide attenuates inflammatory hepcidin by reducing IL-6 secretion and promoting SIRT1-mediated STAT3 deacetylationPubmed:26154696
  • The Impact of Acute Matriptase Inhibition in Hepatic Inflammatory Models.pubmed:27642598
  • In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution.pubmed:27729845
  • Effects of iron supplementation in mice with hypoferremia induced by obesitypubmed:27439539
  • Hepcidin and iron metabolism associated with cardiometabolic risk factors in children: A case–control studypubmed:27139516
  • S-Propargyl-Cysteine, a Novel Hydrogen Sulfide Donor, Inhibits Inflammatory Hepcidin and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway.pubmed:27649298
  • Hepcidin links gluco-toxicity to pancreatic beta cell dysfunction by inhibiting Pdx-1 expressionpubmed:28179377
  • Maternal and cord blood hepcidin levels based on gestational weeks in term and preterm infantspublication:305921433_Maternal_and_cord_blood_hepc
  • Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activationpubmed:29147463
  • Silkworm feces extract improves iron deficiency anemia via suppressing hepcidin expression and promoting iron-regulatory proteins expressionDOI: 10.1039/C7RA09576G
  • Evaluation of a competitive hepcidin ELISA assay in the differential diagnosis of iron deficiency anaemia with concurrent inflammation and anaemia of inflammation in elderly patients10.1186:s12950-017-0166-3
  • AMPK serves as a Therapeutic Target against Anemia of Inflammationpubmed:27923278
  • Micronutrient supplementation in gastric bypass surgery: prospective study on infl ammation and iron metabolism in premenopausal women10.20960/nh.1162
  • Hepcidin suppression in β‑thalassemia is associated with the down‑regulation of atonal homolog 8pubmed:28405918
  • Modulation of iron status biomarkers in tuberculosis-diabetes co-morbidity10.1016/j.tube.2017.11.011
  • The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus.pubmed:28584599
  • The Effects of Angelica Sinensis Polysaccharide on Tumor Growth and Iron Metabolism by Regulating Hepcidin in Tumor-Bearing MicePubmed:29843136
  • inhibits ferroportin translation by modulating FBXL5‐IRP2 axis for its growth within host macrophagesPubmed:29470856
  • Acute and Chronic Iron Overloading Differentially Modulates the Expression of Cellular Iron-homeostatic Molecules in Normal Rat KidneyPubmed:29873589
  • Increased NGAL level associated with iron store in chronic kidney disease with anemiaPubmed:29909502
  • Relationship between obesity and iron deficiency anemia: is there a role of hepcidin?Pubmed:29318945
  • Hepcidin serum levels in HCV chronically mono-infected naïve patients, compared to healthy individuals77856
  • Effect of iron supplement from microalgae on the treatment of iron-deficient anemia in ratsDoi: 10.1039/C8FO01696H
  • New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemiaPubmed: 30792208
  • Inflammatory anemia-associated parameters are related to 28-day mortality in patients with sepsis admitted to the ICU: a preliminary observational studyPubmed: 31183575
  • Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats
  • 3-Amidinophenylalanine-derived matriptase inhibitors can modulate hepcidin production in vitroPubmed: 31659405
  • H2O2 induces oxidative stress damage through the BMP‐6/SMAD/hepcidin axisPubmed: 32012242
  • Increased Hepcidin Levels During a Period of High Training Load Do Not Alter Iron Status in Male Elite Junior RowersPubmed: 32038278
  • Can Calprotectin Show Subclinical Inflammation in Familial Mediterranean Fever Patients?Pubmed: 32174062
  • Iron kinetics following treatment with sucroferric oxyhydroxide or ferric citrate in healthy rats and models of anaemia, iron overload or inflammationPubmed: 32259248
  • Gold-silver core-shell nanoparticle–based impedimetric immunosensor for detection of iron homeostasis biomarker hepcidinPubmed: 33095336
  • Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in …Pubmed: 33065276
  • Relation of serum hepcidin levels and restless legs syndrome in chronic hemodialysis patientsPubmed: 33029690
  • Difference in iron metabolism may partly explain sex-related variability in the manifestation of Wilson's diseasePubmed: 32937238
  • Expression of iron regulatory proteins in full term swine placentaPubmed: 32449967
  • Estrogen deficiency is associated with brain iron deposition via upregulation of hepcidin expression in aged female micePubmed: 32920472
  • Urinary hepcidin concentration in assessment of iron homeostasis in pediatrics
  • COMPARATIVE MONITORING OF THE ENZYME ACTIVITY OF BONE METABOLISM IN PATIENTS AFTER DENTAL IMPLANTATION
  • Serum iron, prealbumin, D-dimer and prothrombin time are indicators of the diagnosis, severity and outcome of Salmonella enterica serovar Enteritidis infection
  • Гепсидин и индекс фиброза печени MELD-XI как маркеры полиорганной недостаточности у пациентов хронической сердечной недостаточностью с …
  • Ассоциации уровней NT-proBNP и гепсидина с клинико-лабораторными параметрами у больных хронической сердечной недостаточностью с разной …
  • Correlation of hepcidin and serum ferritin levels in thalassemia patients at Chiang Mai University Hospital33565577
  • Iron metabolism is disturbed and anti-copper treatment improves but does not normalize iron metabolism in Wilson's disease33555495
  • Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure34139233
  • Continuous Veno-Venous Hemofiltration (CVVH) Improves Iron Metabolism Disorders in Patients With Sepsis: A Single-Center Prospective Cohort Study
  • Evaluation of Serum Cyclooxygenase, Hepcidin Levels in Acute Renal Injury (AKI) Patients Following Cardiac Catheterization
  • High-dose ferric citrate supplementation attenuates omega-3 polyunsaturated fatty acid biosynthesis via downregulating delta 5 and 6 desaturases in rats with high-fat …34787162
  • Caffeine and alcohol-friends or foes of human iron stores?Pubmed:35063815
  • Resveratrol ameliorates iron overload induced liver fibrosis in mice by regulating iron homeostasisPubmed:35698613
  • Serum Calprotectin and Iron Metabolism Biomarker Levels in Behçet's Disease
  • Roles of homopolymeric apoferritin in alleviating alcohol-induced liver injury

Recommend products