CLIA Kit for Indole 3 Acetic Acid (IAA) Pan-species (General) Competition CLIA

IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin

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  • CLIA Kit for Indole 3 Acetic Acid (IAA) Packages (Simulation)
  • CLIA Kit for Indole 3 Acetic Acid (IAA) Packages (Simulation)
  • CLIA Kit for Indole 3 Acetic Acid (IAA) Results demonstration
  • CCA737Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

CLIA Kit for Indole 3 Acetic Acid (IAA)

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Indole 3 Acetic Acid (IAA). A competitive inhibition reaction is launched between biotin labeled Indole 3 Acetic Acid (IAA) and unlabeled Indole 3 Acetic Acid (IAA) (Standards or samples) with the pre-coated antibody specific to Indole 3 Acetic Acid (IAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Indole 3 Acetic Acid (IAA) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Indole 3 Acetic Acid (IAA) level in the sample or standard.

Citations

  • Assisted phytoremediation of mixed metal(loid)-polluted pyrite waste: effects of foliar and substrate IBA application on fodder radishPubMed: 21570105
  • Contribution of indole-3-acetic acid in the plant growth promotion by the rhizospheric strain Bacillus amyloliquefaciens SQR9Springer:Source
  • Structural and metabolic alterations in root systems under limited water conditions in forage grasses of Lolium-Festuca complex
  • Indole-3-Acetic Acid Alleviates Nonalcoholic Fatty Liver Disease in Mice via Attenuation of Hepatic Lipogenesis, and Oxidative and Inflammatory StressPubmed: 31484323

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