CLIA Kit for Inositol Triphosphate (IP3) Pan-species (General) Competition CLIA

InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • CLIA Kit for Inositol Triphosphate (IP3) Packages (Simulation)
  • CLIA Kit for Inositol Triphosphate (IP3) Packages (Simulation)
  • CLIA Kit for Inositol Triphosphate (IP3) Results demonstration
  • CCC037Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Inositol Triphosphate (IP3) and the recovery rates were calculated by comparing the measured value to the expected amount of Inositol Triphosphate (IP3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 89-96 92
heparin plasma(n=5) 87-99 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inositol Triphosphate (IP3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inositol Triphosphate (IP3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Inositol Triphosphate (IP3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-95% 82-97% 94-101% 93-105%
EDTA plasma(n=5) 88-101% 84-104% 80-95% 85-96%
heparin plasma(n=5) 85-93% 92-101% 92-103% 95-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

CLIA Kit for Inositol Triphosphate (IP3)

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Inositol Triphosphate (IP3). A competitive inhibition reaction is launched between biotin labeled Inositol Triphosphate (IP3) and unlabeled Inositol Triphosphate (IP3) (Standards or samples) with the pre-coated antibody specific to Inositol Triphosphate (IP3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inositol Triphosphate (IP3) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Inositol Triphosphate (IP3) level in the sample or standard.

Citations

  • The association between the expression of PAR2 and TMEM16A and neuropathic painPubmed:29257338
  • OSBP-related protein 4L promotes phospholipase Cβ3 translocation from the nucleus to the plasma membrane in Jurkat T-cellsPubmed: 30237164
  • α 1-AR overactivation induces cardiac inflammation through NLRP3 inflammasome activationPubmed: 31530901
  • Exendin-4 inhibits small intestinal glucose sensing and absorption through repression of T1R2/T1R3 sweet taste receptor signalling in streptozotocin diabetic micePubmed:35385790
  • A pyroptosis nanotuner for cancer therapyPubmed:35606443

Recommend products