CLIA Kit for Interleukin 25 (IL25)
IL17E; IL17-E; Interleukin-17E
- UOM
- FOB US$ 588.00 US$ 840.00 US$ 3,780.00 US$ 7,140.00 US$ 58,800.00
- Quantity
Overview
Properties
- Product No.SCB694Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsChemiluminescent immunoassay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryCytokineInfection immunity
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Recovery
Matrices listed below were spiked with certain level of recombinant Interleukin 25 (IL25) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 25 (IL25) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 99-105 | 102 |
EDTA plasma(n=5) | 96-103 | 101 |
heparin plasma(n=5) | 81-98 | 88 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 25 (IL25) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 25 (IL25) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 25 (IL25) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 98-105% | 79-103% | 78-92% | 88-102% |
EDTA plasma(n=5) | 90-101% | 80-101% | 88-97% | 92-101% |
heparin plasma(n=5) | 90-104% | 96-104% | 79-105% | 80-93% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Substrate A | 1×10mL | Substrate B | 1×2mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle
The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 25 (IL25). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 25 (IL25). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 25 (IL25) level in the sample or standard.;
Giveaways
Increment services
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Single-component Reagents of Assay Kit
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Lysis Buffer Specific for ELISA / CLIA
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Quality Control of Kit
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CLIA Kit Customized Service
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Disease Model Customized Service
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Serums Customized Service
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TGFB1 Activation Reagent
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Real Time PCR Experimental Service
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Streptavidin
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Fast blue Protein Stain solution
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Single-component Reagents of FLIA Kit
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Streptavidin-Agarose Beads
Citations
- Molecular analysis of interleukin-25 exons 1 and 2 and its serum levels in Iranian patients with multiple sclerosisPubmed:Pmc4138132
- Epithelial Cell-Derived Cytokines Contribute to the Pathophysiology of Eosinophilic Chronic RhinosinusitisPubmed:26540312
- IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity.pubmed:28194019
- The effect of calprotectin on TSLP and IL-25 production from airway epithelial cellspubmed:27475624
- A mechanism of interleukin-25 production from airway epithelial cells induced by Japanese cedar pollenPubmed:29402661
- Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposisPubmed:30056130
- КЛИНИКО-ЭПИДЕМИОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА И ОСОБЕННОСТИ БАЛЛАНСА ЦИТОКИНОВ ПРИ КЛЕЩЕВОМ ЭНЦЕФАЛИТЕ В …
- effect of Th9/il-9 on the growth of gastric cancer in nude micePubmed: 30988627
- IL‐25 promotes cisplatin resistance of lung cancer cells by activating NF‐κB signaling pathway to increase of major vault proteinPubmed: 31044552
- Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection …
- Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota
- Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus PatientsPubmed:35056005