CLIA Kit for Leukotriene C4 (LTC4) Pan-species (General) Competition CLIA

LT-C4

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • CLIA Kit for Leukotriene C4 (LTC4) Packages (Simulation)
  • CLIA Kit for Leukotriene C4 (LTC4) Packages (Simulation)
  • CLIA Kit for Leukotriene C4 (LTC4) Results demonstration
  • CCA619Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Leukotriene C4 (LTC4) and the recovery rates were calculated by comparing the measured value to the expected amount of Leukotriene C4 (LTC4) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 89-97 94
heparin plasma(n=5) 80-94 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leukotriene C4 (LTC4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leukotriene C4 (LTC4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Leukotriene C4 (LTC4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-89% 87-98% 93-101% 98-105%
EDTA plasma(n=5) 80-105% 82-95% 91-98% 95-102%
heparin plasma(n=5) 88-96% 91-98% 82-93% 96-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

CLIA Kit for Leukotriene C4 (LTC4)

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Leukotriene C4 (LTC4). A competitive inhibition reaction is launched between biotin labeled Leukotriene C4 (LTC4) and unlabeled Leukotriene C4 (LTC4) (Standards or samples) with the pre-coated antibody specific to Leukotriene C4 (LTC4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Leukotriene C4 (LTC4) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Leukotriene C4 (LTC4) level in the sample or standard.

Citations

  • 5-lipoxygenase activation is involved in the mechanisms of chronic hepatic injury in a rat model of chronic aluminum overload exposurepubmed:27368151

Recommend products