CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) Rattus norvegicus (Rat) Sandwich CLIA

CCL20; CKb4; LARC; MIP3-A; SCYA20; ST38; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20; Beta-Chemokine Exodus-1; Liver And Activation-Regulated Chemokine

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  • CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) Packages (Simulation)
  • CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) Packages (Simulation)
  • CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) Results demonstration
  • SCA095Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Macrophage Inflammatory Protein 3 Alpha (MIP3a) and the recovery rates were calculated by comparing the measured value to the expected amount of Macrophage Inflammatory Protein 3 Alpha (MIP3a) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-99 89
EDTA plasma(n=5) 80-90 85
heparin plasma(n=5) 92-99 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Macrophage Inflammatory Protein 3 Alpha (MIP3a) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-97% 93-105% 83-90% 98-105%
EDTA plasma(n=5) 85-104% 80-94% 99-105% 80-102%
heparin plasma(n=5) 83-101% 96-104% 78-104% 96-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Inflammatory Protein 3 Alpha (MIP3a) level in the sample or standard.;

Citations

  • Increased Serum Levels of Macrophage Inflammatory Protein-3α and Cystatin A Predict a Poor Prognosis of Nasopharyngeal CarcinomaPubmed:25396333
  • Serum chemokine network correlates with chemotherapy in non-small cell lung cancerPubMed: 25976768
  • CCR6+ B lymphocytes responding to tumor cell-derived CCL20 support hepatocellular carcinoma progression via enhancing angiogenesis.pubmed:28560063
  • In vitro chemokine (CC motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis10.1186:s40659-018-0161-z
  • Indoleamine 2, 3-Dioxygenase (IDO) Regulates Th17/Treg Immunity in Experimental IgA Nephropathy

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