CLIA Kit for Myelin Basic Protein (MBP) Homo sapiens (Human) Sandwich CLIA

Myelin A1 protein; Myelin membrane encephalitogenic protein

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  • CLIA Kit for Myelin Basic Protein (MBP) Packages (Simulation)
  • CLIA Kit for Myelin Basic Protein (MBP) Packages (Simulation)
  • CLIA Kit for Myelin Basic Protein (MBP) Results demonstration
  • SCA539Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Myelin Basic Protein (MBP) and the recovery rates were calculated by comparing the measured value to the expected amount of Myelin Basic Protein (MBP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-101 95
EDTA plasma(n=5) 78-101 94
heparin plasma(n=5) 89-97 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myelin Basic Protein (MBP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Myelin Basic Protein (MBP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 96-105% 95-103% 94-104%
EDTA plasma(n=5) 86-99% 87-97% 86-94% 92-101%
heparin plasma(n=5) 79-95% 89-103% 85-101% 92-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Myelin Basic Protein (MBP)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Myelin Basic Protein (MBP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Myelin Basic Protein (MBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Myelin Basic Protein (MBP) level in the sample or standard.;

Citations

  • CD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
  • Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat BrainPubmed: 23183024
  • Myelin basic protein and ischemia modified albumin levels in acute ischemic stroke casesPubMed: 26648996
  • Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus)Pubmed:27165445
  • Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomyAA.2016.0008
  • Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord.pubmed:27573615
  • Glial markers and emotional memory in rats following acute cerebral radiofrequency exposures.pubmed:27696165
  • Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomyAA.2016.0008
  • Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosispubmed:28489854
  • Acrolein-mediated neurotoxicity in growing Wistar male ratsPubmed:30033014
  • Association and Risk of Canine Distemper with Respect to Age, Sex and Breed of Dogs Suffering from Demyelinating Neuropathies10.5455:ijlr.20170811080455
  • Biosensors and nanobiosensors for rapid detection of autoimmune diseases: a reviewPubmed: 31760523
  • The role of light desynchronosis in the development of stress-induced aging
  • Association of eosinophil-mediated inflammatory biomarkers with the presence of the Schatzki ring34091432
  • Searching for Noninvasive Predictors of the Diagnosis and Monitoring of Eosinophilic Esophagitis¡ªThe Importance of Biomarkers of the Inflammatory Reaction?¡­
  • Monitoring autoimmune diseases by bioelectrochemical detection of autoantibodies. Application to the determination of anti-myelin basic protein autoantibodies in …Pubmed:35202838

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