CLIA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Canis familiaris; Canine (Dog) Competition CLIA

NT-Pro-BNP; ; N-BNP

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • CLIA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Packages (Simulation)
  • CLIA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Packages (Simulation)
  • CLIA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Results demonstration
  • CCA485Ca.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and the recovery rates were calculated by comparing the measured value to the expected amount of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-104 101
EDTA plasma(n=5) 87-97 93
heparin plasma(n=5) 87-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-98% 83-97% 92-99% 80-99%
EDTA plasma(n=5) 79-93% 90-101% 79-98% 96-105%
heparin plasma(n=5) 78-104% 86-94% 83-102% 94-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

CLIA Kit for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP). A competitive inhibition reaction is launched between biotin labeled N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and unlabeled N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) (Standards or samples) with the pre-coated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) level in the sample or standard.

Citations

  • The role of serum n-terminal pro-brain natriuretic peptide in transient tachypnea of the newbornEuropeanreview: Source
  • Vascular Adhesion Protein-1, a Novel Molecule, in Kidney and Heart Allograft RecipientsPubmed: 23769096
  • Paradoxical low-flow, low-gradient aortic stenosis despite preserved left ventricular ejection fraction: new insights from weights of operatively excised aortic valvesPubmed: 24755006
  • A Randomized Controlled Trial to Study the Effect of Yoga Therapy on Cardiac Function and N Terminal Pro BNP in Heart FailureNCBI: PMC3981569
  • Diabetes enhances the intrahepatic vascular response to endothelin-1 in cirrhotic rats: association with the ETA receptor and pERK up-regulation.Pubmed:24636620
  • Growth-differentiation factor 15 and osteoprotegerin in acute myocardial infarction complicated by cardiogenic shock: a biomarker substudy of the IABP-SHOCK II-trial.Pubmed:24903195
  • Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with HypertensionPubMed: 25747153
  • The Multi-Biomarker Approach for Heart Failure in Patients with HypertensionPubMed: 25984599
  • Effect of Treatment on Body Fluid in Patients with Unilateral Aldosterone Producing Adenoma: Adrenalectomy versus SpironolactonePubMed: 26477337
  • Diabetes enhances the intrahepatic vascular response to endothelin‐1 in cirrhotic rats: association with the ETA receptor and pERK up‐regulationPubMed: 24636620
  • Qiliqiangxin improves cardiac function and attenuates cardiac remodeling in rats with experimental myocardial infarctionPubMed: 26261541
  • NT-proBNP in Children With Left to Right Shunt and Dilated Cardiomyopathyserve:4485
  • Human myoblast transplantation in mice infarcted heart alters the expression profile of cardiac genes associated with left ventricle remodelingPubmed:26457413
  • Cardiovascular changes in patients with non-severe Plasmodium vivax malariaarticle:S2352-9067(16)30010-0
  • Soluble Glycoprotein 130 and Heat Shock Protein 27 as Novel Candidate Biomarkers of Chronic Heart Failure with Preserved Ejection FractionPubmed:27067668
  • Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies.pubmed:27448535
  • Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure.pubmed:28565777
  • Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure.pubmed:28127817
  • Study of Vitamin D Status in Patients with Dilated Cardiomyopathy at a Teaching Hospital in North India.pubmed:28465969
  • 5-methoxytryptophan is a potential marker for post-myocardial infarction heart failure - apreliminary approach to clinical utility.pubmed:27526355
  • Beneficial Effects of Ozone Therapy on Oxidative Stress, Cardiac Functions and Clinical Findings in Patients with Heart Failure Reduced Ejection Fractionpubmed:28097518
  • Serial heart rhythm complexity changes in patients with anterior wall ST segment elevation myocardial infarction.pubmed:28252107
  • The predictive capabilities of a novel cardiovascular magnetic resonance derived marker of cardiopulmonary reserve on established prognostic surrogate markers in patients with pulmonary vascular disease: results of a longitudinal pilot studypubmed:28065166
  • AVALIAÇÃO ELETROCARDIOGRÁFICA AMBULATORIAL DE CÃES COM EHRLIQUIOSE MONOCÍTICA CRÔNICAhandle:11449
  • Cardiac biomarkers in dogs with visceral leishmaniasisS0301-732X2016000300004
  • Evaluation of N-Terminal Pro Brain Natriuretic Peptide as a biomarker for clinical severity of heart failure in pediatric populationAttach432
  • Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathwaypubmed:28698735
  • QSKL protects against myocardial apoptosis on heart failure via PI3K/Akt-p53 signaling pathway.pubmed:29209026
  • Reversal effects of low-dose imatinib compared with sunitinib on monocrotaline-induced pulmonary and right ventricular remodeling in ratspubmed:29100963
  • Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Ratspubmed:28706558
  • Prediction of electro-anatomical substrate using APPLE score and biomarkersPubmed:29893827
  • Role of NT-proANP and NT-proBNP in patients with atrial fibrillation: Association with atrial fibrillation progression phenotypesPubmed:29604419
  • MicroRNAs in Peripheral Mononuclear Cells as Potential Biomarkers in Hypertensive Patients With Heart Failure With Preserved Ejection FractionPubmed:29506053
  • Differential microRNA expression profiles and bioinformatics analysis between young and aging spontaneously hypertensive ratsPubmed:29328372
  • Regulation of circulating chromogranin B levels in heart failurePubmed:29098879
  • Effect of 6-min Walk Test on pro-BNP Levels in Patients with Pulmonary Arterial HypertensionPubmed:29564533
  • Dynamic monitoring the level of NT-proBNP in assessing the value of cardiac function and prognosis in patients with systemic infectionijcem0072283.pdf
  • Signature-oriented investigation of the efficacy of multicomponent drugs against heart failurePubmed: 30230922
  • Qiliqiangxin ameliorates cardiac dysfunction in heart failure rats post myocardial infarction via activation of HIF/VEGF pathway
  • Decreasing cardiac iron overload with Amlodipine and Spirulina in children with β-thalassemiaDoi: 10.1016/j.phoj.2018.10.001
  • Acute Responses of Novel Cardiac Biomarkers to a 24-h Ultra-MarathonPubmed: 30625976
  • Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure.
  • Biological markers for the effects of yoga as a complementary and alternative medicinePubmed: 30735481
  • Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling PathwayPubmed: 30890937
  • Potential Effects of CXCL9 and CCL20 on Cardiac Fibrosis in Patients with Myocardial Infarction and Isoproterenol-Treated RatsPubmed: 31083544
  • Evaluation of heart rate variability and behavior of electrocardiographic parameters in dogs affected by chronic Monocytic EhrlichiosisPubmed: 31125348
  • Cardio-protective effect of the secretome of Sca-1+ and Sca-1-cells in heart failure–not equal, but equally important? Paracrine cell effects on heart failure
  • The potential role of inflammation in cryptogenic stroke
  • Arterial Stiffness and Indices of Left Ventricular Diastolic Dysfunction in Patients with Embolic Stroke of Undetermined EtiologyPubmed: 31612072
  • Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle HypertrophyPubmed: 32148580
  • Preclinical development of a miR-132 inhibitor for heart failure treatmentPubmed: 32005803
  • Evaluating the Relationship of GDF-15 with Clinical Characteristics, Cardinal Features, and Survival in Multiple MyelomaPubmed: 33144847
  • CDR132L improves systolic and diastolic function in a large animal model of chronic heart failurePubmed: 33089304
  • Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult micePubmed: 33068716
  • Apela improves cardiac and renal function in mice with acute myocardial infarctionPubmed: 32686917
  • Left Ventricular Diastolic Dysfunction as Predictor of Unfavorable Prognosis After ESUS33727824
  • κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a34373768
  • Hemodynamic Responses in Lower Limb Lymphedema Patients Undergoing Physical Therapy34356497
  • Risk Stratification of Pulmonary Thromboembolism using Brain Natriuretic Peptide and Troponin I; a Brief ReportPubmed:35072097
  • Interplay between mineral bone disorder and cardiac damage in acute kidney injury: from Ca2+ mishandling and preventive role of Klotho in mice to its potential …Pubmed:35077866
  • The Key Role of Hepcidin-25 in Anemia in Multiple Myeloma Patients with Renal ImpairmentPubmed:35334593
  • Prothymosin α Gene Transfer Modulates Myocardial Remodeling after Ischemia-Reperfusion InjuryPubmed:35273440

Recommend products