CLIA Kit for Programmed Cell Death Protein 1 Ligand 1 (PDL1) Homo sapiens (Human) Sandwich CLIA

CD274; PDCD1LG1; B7-H; B7H1; PD-L1; PDCD1L1; PDCD1-LG1; Programed Death Ligand 1

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  • CLIA Kit for Programmed Cell Death Protein 1 Ligand 1 (PDL1) Packages (Simulation)
  • CLIA Kit for Programmed Cell Death Protein 1 Ligand 1 (PDL1) Packages (Simulation)
  • CLIA Kit for Programmed Cell Death Protein 1 Ligand 1 (PDL1) Results demonstration
  • SCA788Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Programmed Cell Death Protein 1 Ligand 1 (PDL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Programmed Cell Death Protein 1 Ligand 1 (PDL1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-98 90
EDTA plasma(n=5) 80-101 86
heparin plasma(n=5) 90-105 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 1 (PDL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 1 (PDL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Programmed Cell Death Protein 1 Ligand 1 (PDL1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-105% 97-105% 94-103% 97-104%
EDTA plasma(n=5) 89-96% 80-89% 98-105% 92-99%
heparin plasma(n=5) 93-101% 89-96% 88-102% 80-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Programmed Cell Death Protein 1 Ligand 1 (PDL1)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 1 Ligand 1 (PDL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 1 Ligand 1 (PDL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Programmed Cell Death Protein 1 Ligand 1 (PDL1) level in the sample or standard.;

Citations

  • Level of circulating PD-L1 expression in patients with advanced gastric cancer and its clinical implicationsNCBI: PMC3937742
  • High level of soluble programmed cell death ligand 1 in blood impacts overall survival in aggressive diffuse large B-Cell lymphoma: results from a French multicenter clinical trial.Pubmed:24732592
  • PD‐L1 gene polymorphisms and low serum level of PD‐L1 protein are associated to type 1 diabetes in ChilePubmed:24816853
  • Ratios of T-cell immune effectors and checkpoint molecules as prognostic biomarkers in diffuse large B-cell lymphoma: a population-based studyPubMed: 26686046
  • High levels of the soluble programmed death-ligand (sPD-L1) identify hepatocellular carcinoma patients with a poor prognosis.Pubmed:27039170
  • Serum levels of soluble programmed cell death ligand 1 as a prognostic factor on the first-line treatment of metastatic or recurrent gastric cancerarticle:10.1007
  • PD-L1 is upregulated by EBV-driven LMP1 through NF-κB pathway and correlates with poor prognosis in natural killer/T-cell lymphomapubmed:27737703
  • High plasma levels of soluble programmed cell death ligand 1 are prognostic for reduced survival in advanced lung cancer.pubmed:28212990
  • Soluble programmed death-ligand 1 (sPDL1) and neutrophil-to-lymphocyte ratio (NLR) predictssurvival in advanced biliary tract cancer patients treated with palliative chemotherapy.pubmed:27780932
  • High post-treatment serum levels of soluble programmed cell death ligand 1 predict earlyrelapse and poor prognosis in extranodal NK/T cell lymphoma patients.pubmed:27105512
  • Soluble PD-L1: A biomarker to predict progression of autologous transplantation in patients with multiple myelomapubmed:27566569
  • Programmed death-ligand 1 and its soluble form are highly expressed in nasal natural killer/T-cell lymphoma: a potential rationale for immunotherapy.pubmed:28349165
  • Soluble programmed death-ligand 1 as a prognostic biomarker for overall survival in patients with diffuse large B-cell lymphoma: a replication study and combined analysis of 508 patientsleu2016385a
  • Plasma levels of soluble programmed death ligand-1 may be associated with overall survival in nonsmall cell lung cancer patients receiving thoracic radiotherapy.pubmed:28207525
  • Inverse correlation of soluble programmed cell death-1 ligand-1 (sPD-L1) with eosinophil count and clinical severity in allergic rhinitis patientspubmed:27617656
  • Plasma levels of soluble programmed death ligand-1 may be associated with overall survival in nonsmall cell lung cancer patients receiving thoracic radiotherapyPMC5319514
  • Soluble Programed Death Receptor 1 Ligand (sPD-L1) in Pleural Fluid of Patients with Malignant Pleural Mesothelioma (MPM)Pdf:10.1164
  • Soluble Programmed Cell Death Ligand 1 as a Novel Biomarker for Nivolumab Therapy for Non–Small-cell Lung CancerPubmed:29859759
  • Increased levels of soluble programmed death ligand 1 associate with malignancy in patients with dermatomyositisPubmed:29419471
  • High Serum Level of Soluble Programmed Death Ligand 1 is Associated With a Poor Prognosis in Hodgkin LymphomaPubmed:29698935
  • Increased levels of soluble co-stimulatory molecule PD-L1 (B7-H1) in the plasma of viraemic HIV-1+ individualsPubmed: 30236481
  • Pre-treatment serum levels of soluble programmed cell death-ligand 1 predict prognosis in patients with hepatitis B-related hepatocellular carcinomaPubmed: 30267213
  • Soluble PD-L1 Expression in Circulation as a Predictive Marker for Recurrence and Prognosis in Gastric Cancer: Direct Comparison of the Clinical Burden Between …Pubmed: 30565045
  • Identification of programmed cell death 1 and its ligand in the testicular tissue of micePubmed: 30578744
  • Clinical significance of soluble programmed cell death-1 and soluble programmed cell death-ligand 1 in patients with locally advanced rectal cancer treated …Pubmed: 30807610
  • Elevated levels of soluble PD-L1 are associated with reduced recurrence in papillary thyroid cancerPubmed: 31252406
  • Prognostic implications of soluble programmed death-ligand 1 and its dynamics during chemotherapy in unresectable pancreatic cancerPubmed: 31366979
  • The Clinical Significance of Soluble PD-1 and PD-L1 in Lung CancerPubmed: 31675543
  • Clinical implications of APOBEC3A and 3B expression in patients with breast cancerPubmed: 32176735
  • Predictive Value of Soluble PD-1, PD-L1, VEGFA, CD40 Ligand and CD44 for Nivolumab Therapy in Advanced Non-Small Cell Lung Cancer: A Case-Control StudyPubmed: 32085544
  • Serum levels of soluble programmed death-ligand 1 (sPD-L1) in patients with primary central nervous system diffuse large B-cell lymphomaPubmed: 32054467
  • The prognostic role of soluble transforming growth factor‐β and its correlation with soluble programmed death‐ligand 1 in biliary tract cancerPubmed: 32780918
  • Prognostic impacts of tumoral expression and serum levels of PD-L1 and CTLA-4 in colorectal cancer patientsPubmed: 32577816
  • Soluble PD-L1: a potential immune marker for HIV-1 infection and virological failurePubmed: 32443313
  • The clinical implication of soluble PD-L1 (sPD-L1) in patients with breast cancer and its biological function in regulating the function of T lymphocyte33688997
  • Prognostic prospect of soluble programmed cell death ligand-1 (sPD-L1) in cancer management34180502
  • Microglial PD‐1 stimulation by astrocytic PD‐L1 suppresses neuroinflammation and Alzheimer's disease pathology34825707
  • A Novel Small Cyclic Peptide-Based 68Ga-Radiotracer for Positron Emission Tomography Imaging of PD-L1 Expression in Tumors34910492
  • Macrophage Associated Immune Checkpoint CD47 Blocking Ameliorates EndometriosisPubmed:35404426
  • Microbial hydrogen “manufactory” for enhanced gas therapy and self-activated immunotherapy via reduced immune escapePubmed:35705974
  • Assessment of the RANTES Level Correlation and Selected Inflammatory and Pro-Angiogenic Molecules Evaluation of Their Influence on CRC Clinical Features: A …Pubmed:35208526

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