CLIA Kit for Semaphorin 3A (SEMA3A)

Matrices listed below were spiked with certain level of recombinant Semaphorin 3A (SEMA3A) and the recovery rates were calculated by comparing the measured value to the expected amount of Semaphorin 3A (SEMA3A) in samples.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Semaphorin 3A (SEMA3A) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The microplate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3A (SEMA3A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Semaphorin 3A (SEMA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Semaphorin 3A (SEMA3A) level in the sample or standard.;