CLIA Kit for Thrombospondin 2 (THBS2)

TSP2

UOM
1. 48T
2. 96T
3. 96T*5
4. 96T*10
5. 96T*100
FOB US$ 605.00 US$ 864.00 US$ 3,888.00 US$ 7,344.00 US$ 60,480.00
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Overview

Properties

Product No.SCD822Mu
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsChemiluminescent immunoassay for Antigen Detection.
Research use only
DownloadInstruction Manual
CategoryHematology

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Packages (Simulation)
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Results demonstration

Typical Standard Curve

ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Thrombospondin 2 (THBS2) and the recovery rates were calculated by comparing the measured value to the expected amount of Thrombospondin 2 (THBS2) in samples.

Matrix	Recovery range (%)	Average(%)
sodium citrate plasma(n=5)	85-103	94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thrombospondin 2 (THBS2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thrombospondin 2 (THBS2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thrombospondin 2 (THBS2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
sodium citrate plasma(n=5)	84-98%	78-103%	95-103%	89-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
Substrate A	1×10mL	Substrate B	1×2mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Thrombospondin 2 (THBS2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thrombospondin 2 (THBS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thrombospondin 2 (THBS2) level in the sample or standard.;