CLIA Kit for Thyroid Hormone Responsive (THRSP) Homo sapiens (Human) Sandwich CLIA

S14; SPOT14; LPGP1; Lipogenic Protein 1; Thyroid hormone-inducible hepatic protein; Spot 14 protein

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  • CLIA Kit for Thyroid Hormone Responsive (THRSP) Packages (Simulation)
  • CLIA Kit for Thyroid Hormone Responsive (THRSP) Packages (Simulation)
  • CLIA Kit for Thyroid Hormone Responsive (THRSP) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Thyroid Hormone Responsive (THRSP) and the recovery rates were calculated by comparing the measured value to the expected amount of Thyroid Hormone Responsive (THRSP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-94 91
EDTA plasma(n=5) 78-102 97
heparin plasma(n=5) 79-90 84

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroid Hormone Responsive (THRSP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroid Hormone Responsive (THRSP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thyroid Hormone Responsive (THRSP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-104% 81-99% 78-101% 97-105%
EDTA plasma(n=5) 84-92% 88-101% 81-101% 80-101%
heparin plasma(n=5) 90-101% 92-103% 81-102% 90-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Thyroid Hormone Responsive (THRSP)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Thyroid Hormone Responsive (THRSP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thyroid Hormone Responsive (THRSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thyroid Hormone Responsive (THRSP) level in the sample or standard.;

Citations

  • Comparative thyroid disruption by o, p'-DDT and p, p'-DDE in zebrafish embryos/larvae
  • Thyroid disruption and developmental toxicity caused by triphenyltin (TPT) in zebrafish embryos/larvaePubmed: 32173372

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