CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)
SFD; TIMP Metallopeptidase Inhibitor 3; Metallopeptidase Inhibitor 3; Sorsby Fundus Dystrophy; Pseudoinflammatory
- UOM
- FOB US$ 638.00 US$ 912.00 US$ 4,104.00 US$ 7,752.00 US$ 63,840.00
- Quantity
Overview
Properties
- Product No.SCA129Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- ApplicationsChemiluminescent immunoassay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryTumor immunityCardiovascular biology
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Recovery
Matrices listed below were spiked with certain level of recombinant Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) and the recovery rates were calculated by comparing the measured value to the expected amount of Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 95-103 | 101 |
EDTA plasma(n=5) | 79-95 | 89 |
heparin plasma(n=5) | 94-102 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 81-92% | 86-93% | 80-97% | 84-98% |
EDTA plasma(n=5) | 90-103% | 79-96% | 88-104% | 96-104% |
heparin plasma(n=5) | 89-101% | 92-101% | 91-99% | 82-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
Substrate A | 1×10mL | Substrate B | 1×2mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle
The microplate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) level in the sample or standard.;
Giveaways
Increment services
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Single-component Reagents of Assay Kit
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Lysis Buffer Specific for ELISA / CLIA
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Quality Control of Kit
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CLIA Kit Customized Service
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Disease Model Customized Service
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Serums Customized Service
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TGFB1 Activation Reagent
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Streptavidin
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Fast blue Protein Stain solution
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Single-component Reagents of FLIA Kit
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Streptavidin-Agarose Beads
Citations
- Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis VirusPubmed: source
- Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cellsRsmjournals: Source
- A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
- The unwounded skin remodeling in animal models of diabetes types 1 and 2.Pubmed: 24020818
- Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infectionPubmed:26925446
- Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells.pubmed:27890915
- Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancerpubmed:28421310
- Circulating and tissue IMP3 levels are correlated with poor survival in renal cell carcinomaPubmed: 30650187
- Altered TIMP-3 Levels in the Cerebrospinal Fluid and Plasma of Patients with Alzheimer's DiseasePubmed:35629249