CLIA Kit for Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) Homo sapiens (Human) Sandwich CLIA

CD354; Triggering Receptor Expressed On Monocytes-1

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  • CLIA Kit for Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) Packages (Simulation)
  • CLIA Kit for Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) Packages (Simulation)
  • CLIA Kit for Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) Results demonstration
  • SCA213Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) and the recovery rates were calculated by comparing the measured value to the expected amount of Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-101 95
EDTA plasma(n=5) 95-103 98
heparin plasma(n=5) 80-90 84

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-105% 85-97% 78-91% 95-102%
EDTA plasma(n=5) 88-95% 98-105% 93-101% 84-99%
heparin plasma(n=5) 81-88% 88-104% 89-102% 95-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Triggering Receptor Expressed On Myeloid Cells 1 (TREM1)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Triggering Receptor Expressed On Myeloid Cells 1 (TREM1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Triggering Receptor Expressed On Myeloid Cells 1 (TREM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Triggering Receptor Expressed On Myeloid Cells 1 (TREM1) level in the sample or standard.;

Citations

  • Successful Treatment of Acute Lung Injury with Pitavastatin in Septic Mice: Potential Role of Glucocorticoid Receptor Expression in Alveolar MacrophagesAspet: 171462
  • Vitamin D attenuates inflammation, fatty infiltration, and cartilage loss in the knee of hyperlipidemic microswine.pubmed:27624724
  • Role of Triggering Receptor Expressed on Myeloid Cell-1 Expression in Mammalian Target of Rapamycin Modulation of CD8+ T-cell Differentiation during the Immune Response to Invasive Pulmonary AspergillosisPMC5443028
  • Increased TLR4 and TREM-1 expression on monocytes and neutrophils in preterm birth: further evidence of a proinflammatory statePubmed:29534643
  • A promising novel biomarker for early-onset preeclampsia: soluble trigger receptor expressed on myeloid cells-1 (sTREM-1)Pubmed: 33207989
  • Ioannis Gkougkourellas, Alexandros Sarantopoulos, Konstantinos Tselios, Athanasios Kalogeridis

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