CLIA Kit for Triiodothyronine (T3) Pan-species (General) Competition CLIA

3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

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  • CLIA Kit for Triiodothyronine (T3) Packages (Simulation)
  • CLIA Kit for Triiodothyronine (T3) Packages (Simulation)
  • CLIA Kit for Triiodothyronine (T3) Results demonstration
  • CCA453Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Triiodothyronine (T3) and the recovery rates were calculated by comparing the measured value to the expected amount of Triiodothyronine (T3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-104 101
EDTA plasma(n=5) 96-105 99
heparin plasma(n=5) 87-94 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Triiodothyronine (T3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Triiodothyronine (T3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Triiodothyronine (T3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-91% 88-102% 82-104% 81-96%
EDTA plasma(n=5) 78-101% 83-91% 94-102% 92-102%
heparin plasma(n=5) 91-98% 95-105% 95-104% 87-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.

CLIA Kit for Triiodothyronine (T3)

Test principle

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Triiodothyronine (T3). A competitive inhibition reaction is launched between biotin labeled Triiodothyronine (T3) and unlabeled Triiodothyronine (T3) (Standards or samples) with the pre-coated antibody specific to Triiodothyronine (T3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Triiodothyronine (T3) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Triiodothyronine (T3) level in the sample or standard.

Citations

  • Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvaeScienceDirect: S0166445X12000215
  • Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axisPubMed: 23220413
  • Effect of dietary tryptophan on plasma growth hormone and thyroid hormone in broiler chicksScholarsresearchlibrary: Source
  • Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studiesLifesciencesite: Source
  • A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism Pubmed: 23468891
  • Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay.ScienceDirect: S0166445X13002129
  • Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvaePubmed:25456233
  • Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam Ruditapes philippinarumscience:S0045653516302016
  • Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspringpubmed:27803384
  • Exposure of pregnant mice to triclosan impairs placental development and nutrient transport.pubmed:28322267
  • Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus).pubmed:28187359
  • Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disordershandle:123456789
  • Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes09291016.2016.1275393
  • Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reductionpubmed:28973666
  • Transgenerational endocrine disruption and neurotoxicity in zebrafish larvae after parental exposure to binary mixtures of decabromodiphenyl ether (BDE-209) and lead10.1016/j.envpol.2017.06.053
  • 四溴双酚A对菲律宾蛤仔的生长抑制效应机理Growth inhibition Effects of TBBPA on juvenile manila clam Ruditapes philippinarum5790267088906950000
  • Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats10.4314/tjpr.v16i5.9
  • Screening thyroid hormone disrupting effects of benzophenones using GH3, FRTL-5 cells and zebrafish10371/137708
  • Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult10371/137708
  • Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish.pubmed:28624628
  • Thyroid hormone-disrupting potentials of major benzophenones in two cell lines (GH3 and FRTL-5) and embryo-larval zebrafishPubmed:29995391
  • Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island populationPubmed:29525625
  • Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons10.1093:toxsci
  • Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in MicePubmed:29403355
  • Activation of 5-HT1A receptors in the hypothalamic paraventricular nuclei (PVN) negatively regulates cytochrome P450 expression and activity in rat liverPubmed:29555828
  • Age-related changes in testicular parameters and their relationship to thyroid hormones and testosterone in male Murrah buffaloes10.5194:aab-61-191-2018
  • Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition:
  • Transgenerational thyroid endocrine disruption induced by bisphenol S affects the early development of zebrafish offspringPubmed: 30243188
  • Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in MicePubmed: 30404008
  • Serotonin receptors of 5-HT2 type in the hypothalamic arcuate nuclei (ARC) positively regulate liver cytochrome P450 via stimulation of the growth hormone-releasing …Pubmed: 30518657
  • Parental exposure to 6: 2 chlorinated polyfluorinated ether sulfonate (F53B) induced transgenerational thyroid hormone disruption in zebrafishDoi: 10.1016/j.scitotenv.2019.02.198
  • Thyroid hormone profiles and TSH evaluation during early pregnancy and the transition period in dairy cowsPubmed: 30784791
  • Inhibition of growth in juvenile manila clam Ruditapes philippinarum: Potential adverse outcome pathway of TBBPAPubmed: 30844590
  • Effects of cis-bifenthrin enantiomers on the growth, behavioral, biomarkers of oxidative damage and bioaccumulation in Xenopus laevisPubmed: 31276910
  • Exposure to humidifier disinfectants induces developmental effects and disrupts thyroid endocrine systems in zebrafish larvaePubmed: 31539807
  • MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression ModelPubmed: 31540304
  • Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signalingPubmed: 31809938
  • Therapeutic effect of mesenchymal stem cell on Hashimoto's thyroiditis in a rat model by modulating Th17/Treg cell balancePubmed: 31793369
  • Interaction between hypoxia and perfluorobutane sulfonate on developmental toxicity and endocrine disruption in marine medaka embryosPubmed: 32172180
  • Effect of triadimefon and its metabolite on adult amphibians Xenopus laevisPubmed: 31743868
  • Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axisPubmed: 33177714
  • Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio)Pubmed: 32525014
  • Changes in thyroid hormone levels and related gene expressions in embryo–larval zebraish exposed to binary combinations of bifenthrin and acetochlorPubmed: 32468518
  • Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposição materna ao triclosan causa …
  • Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio)
  • Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine©\treated rat dams during pregnancy and lactation33433934
  • High levels of thyroid hormone impair regulatory T cells function via reduced PD-1 expression33758937
  • Genetic Manipulation on Zebrafish duox Recapitulate the Clinical Manifestations of Congenital Hypothyroidism34019632
  • Xenopus laevis tadpoles exposed to metamifop: Changes in growth, behavioral endpoints, neurotransmitters, antioxidant system and thyroid development34126306
  • Exposure of adult zebrafish (Danio rerio) to Tetrabromobisphenol A causes neurotoxicity in larval offspring, an adverse transgenerational effect33647619
  • Improved Therapeutic Efficiency against Obesity through Transdermal Drug Delivery Using Microneedle Arrays
  • The Selective NMDA Receptor GluN2B Subunit Antagonist CP-101,606 with Antidepressant Properties Modulates Cytochrome P450 Expression in the Liver34683936
  • Thyroid disruption and growth inhibition of zebrafish embryos/larvae by phenanthrene treatment at environmentally relevant concentrations34933138
  • Probiotics inhibit the stunted growth defect of perfluorobutanesulfonate via stress and thyroid axes in zebrafish larvae34428700
  • Perfluorohexanoic acid caused disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvaePubmed:35131581
  • Environmental Co-Exposure to Potassium Perchlorate and Cd Caused Toxicity and Thyroid Endocrine Disruption in Zebrafish Embryos and Larvae (Danio rerio)Pubmed:35448459

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