Polyclonal Antibody to Nitrotyrosine (NT)
3-Nitro-L-Tyrosine; 3-Nitrotyrosine
Overview
Properties
- Product No.PAB863Ge01
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayInfection immunityRheumatology
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen CPB863Ge11-BSA Conjugated Nitrotyrosine (NT)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 0.5mg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against NT. It has been selected for its ability to recognize NT in ELISA and CLIA.
Usage
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
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Citations
- 3-Nitrotyrosine, a biomarker for cardiomyocyte apoptosis induced by diabetic cardiomyopathy in a rat modelPubmed: 23970331
- Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitisPubmed:24992835
- Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in RatsPubMed: 26385569
- Protective effects of Telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuro-inflammation and amyloidogenesis: possible role of brain derived neurotrophic factor10.1139:cjpp-2017-0042
- The cardiovascular phenotype of adult patients with phenylketonuriaPubmed: 31492166
- The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice33303990