Biotin-Linked Polyclonal Antibody to Testosterone (Testo)
T; Testesteron; Testeronun
Overview
Properties
- Product No.LAA458Ge71
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryEndocrinologyReproductive scienceHormone metabolism
- SourceAntibody labeling
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody PAA458Ge01-Polyclonal Antibody to Testosterone (Testo)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Specifity
The antibody is a rabbit polyclonal antibody raised against Testo. It has been selected for its ability to recognize Testo in immunohistochemical staining and western blotting.
Usage
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
Citations
- Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature RatsJstage: source
- Insulin Receptor Substrate 2 Is Required for Testicular DevelopmentPubMed: PMC3669358
- Central effects of camphor on GnRH and sexual hormones in male ratPubmed: 24551777
- Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells Pubmed: 23954356
- Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in RatsPubmed: 24051982
- The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats.Pubmed: 24642156
- Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineeringRsc: Source
- Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesisScienceDirect: S0006291X14001843
- Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostatePubmed:Pmc4100933
- Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspringPubmed:24126190
- The impact of maternal consumption of cafeteria diet on reproductive function in the offspringPubmed:24631302
- The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in ratsPubmed:24642156
- Perfluorooctane sulfonate effects on the reproductive axis in adult male ratsPubmed:25171141
- Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model.Pubmed:25185055
- Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertilityPubmed:25505846
- Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapyTurkishjournalofurology:Source
- Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetesPubMed: 26379831
- Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult RatsPubMed: 26200553
- Plasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic ExtractAensiweb: Aeb
- Elevated Serum Bisphenol A Level in Patients with Dilated CardiomyopathyPubMed: 25996886
- Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis periodPubMed: 25782374
- Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarctionPubMed: 25770118
- Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrhscience:S2211383516301812
- Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t MicrobiotaPubmed:27093642
- Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle countPubmed:26948295
- Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARαPubmed:27413362
- THE EFFECT OF KISSPEPTIN ON SPERMATOGENESIS AND APOPTOSIS IN RATSfileID:693211
- Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in MicePubmed:26771609
- Molecular characterization of insulin resistance and glycolytic metabolism in the rat uteruspubmed:27461373
- Maternal Sleep Deprivation Alters Reproductive Capability of Male Offspring in Wistar RatsAkindele922016JALSI29782.pdf
- Recovery of fertility in quinestrol-treated or diethylstilbestrol-treated mice: Implications for rodent management.pubmed:27611741
- Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.pubmed:28336389
- Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum.pubmed:27838061
- Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARαpubmed:27413362
- Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats.pubmed:28000943
- The effect of kisspeptin on spermatogenesis and apoptosis in rats.pubmed:28263511
- The Angiogenic Effect of Testosterone Supplementation on Brain of Aged Mice.13006045
- Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitiumS2405580816300991
- Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndromepubmed:28673204
- Toxic effects of Tripterygium wilfordii Hook F on the reproductive system of adolescent male ratspubmed:28938525
- Testosterone deficiency prevents left ventricular contractility dysfunction after myocardial infarction pubmed:28606867
- The effect of testosterone supplementation on morphometry and VEGF expression level of thoracic aorta in aged miceDOI: 10.1501/Vetfak_0000002791
- Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-)pubmed:28506466
- Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathwaypubmed:29020999
- Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island populationPubmed:29525625
- Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrationsPubmed:29775925
- deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and …Pubmed:29411926
- DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathwayPubmed:29321035
- Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) …Pubmed:29627845
- Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes …10.3892:ol.2018.9165
- Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neuronsDoi: 10.1016/j.biopha.2018.08.135
- Resveratrol attenuates metabolic, sperm, and testicular changes in adult Wistar rats fed a diet rich in lipids and simple carbohydratesPubmed: 30198494
- 基因敲除损伤小鼠睾丸睾酮产生与生精功能
- Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in RatsPubmed: 30569668
- Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients
- Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndromePubmed: 29535146
- Brain‐derived neurotrophic factor: A steroidogenic regulator of Leydig cellsPubmed: 30628054
- Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary
- iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine
- The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.
- The Effect of Testosterone Supplementation on Capillar Morphometry and VEGF Expression Level of the Heart in Aged Mice
- Cyclophilin D participates in the inhibitory effect of high‐fat diet on the expression of steroidogenic acute regulatory proteinPubmed: 31373170
- Revisiting the relationships of 2D:4D with androgen receptor (AR) gene and current testosterone levels: Replication study and meta‐analysesPubmed: 31359506
- Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male ratsPubmed: 31353623
- Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signalingPubmed: 31809938
- Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cellsPubmed: 32334352
- Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of TreatmentPubmed: 33159671
- Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance …Pubmed: 33109277
- Novel antihypertensive action of rutin is mediated via inhibition of angiotensin converting enzyme/mineralocorticoid receptor/angiotensin 2 type 1 receptor (ATR1) …Pubmed: 33089540
- Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio)Pubmed: 33011509
- MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistancePubmed: 32757043
- Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic …Pubmed: 32698821
- Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal ModelPubmed: 32517356
- Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at pubertyPubmed: 32478927
- N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in ratsPubmed: 32447106
- Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
- Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS
- IVERMECTINA IMPEDE DANOS TESTICULARES EM RATOS
- The effects of gold nanoparticles on Leydig cells and male reproductive function in mice
- The Effect of Acupuncture on MiRNA Expression Profiles in the Ovaries of Rats with PCOS and Insulin Resistance
- BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose33508368
- Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion33965371
- A comparative study on the aphrodisiac activity of food plants< i> Mondia whitei, Chenopodium album, Ccucurbita pe...
- MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 134637688
- Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice34730391
- Yohimbine hydrochloride inhibits benign prostatic hyperplasia by downregulating steroid 5α-reductase type 234265299
- Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with …Pubmed:35063921
- Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in MicePubmed:35257354