Monoclonal Antibody to Cyanocobalamin (CNCbl) Pan-species (General) Monoclonal antibody

VB12; Vitamin B12; Cobalamin; α-5,6-Dimethylbenzimidazolyl Cobamidcyanide

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number C3
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen CPA924Ge11-BSA Conjugated Cyanocobalamin (CNCbl)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Cyanocobalamin (CNCbl) Packages (Simulation)
  • Monoclonal Antibody to Cyanocobalamin (CNCbl) Packages (Simulation)
  • Monoclonal Antibody to Cyanocobalamin (CNCbl) competitive inhibition ELISA;
    Coated protein:CNCbl conjugated to OVA (Catalog:CPA924Ge21);
    Standard:Series Diluted CNCbl;
    Primary Ab: 0.2µg/ml Mouse Anti CNCbl Antibody;
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-mouse IgG
    PolyclonalAntibody
    (Catalog: SAA544Rb19)
    IC50=0.2ng/ml
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CNCbl. It has been selected for its ability to recognize CNCbl in ELISA and CLIA.

Usage

Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Vitamin Status and the Development of Postoperative Cognitive Decline in Elderly Surgical Oncologic Patients.pubmed:29058145
  • Selected Risk Nutritional Factors for Chemotherapy-Induced Polyneuropathy.pubmed:28587059
  • Lettore di micropiastre multimodale:
  • Vitamin A, C, D, E and B12 Levels in Leprosy: A Case Control Study
  • Folate, Vitamin B12, and Homocysteine Levels in Women With Neural Tube Defect-Affected Pregnancy in Addis Ababa, EthiopiaPubmed:35464038

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