Monoclonal Antibody to Vitamin D3 (VD3)
Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol
Overview
Properties
- Product No.MAA920Ge21
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayReproductive scienceNutrition metabolismBone metabolism
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG2b Kappa, Clone Number C1
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen CPA920Ge21-OVA Conjugated Vitamin D3 (VD3)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Specifity
The antibody is a mouse monoclonal antibody raised against VD3. It has been selected for its ability to recognize VD3 in immunohistochemical staining and western blotting.
Usage
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Pan-species (General)Giveaways
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Citations
- Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Ratsprofile:303311214
- Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats:
- Deficiencia de vitamina D en madres y neonatos mexicanospubmed:29099117
- P38/TRHr-Dependent Regulation of TPO in Thyroid Cells Contributes to the Hypothyroidism of Triclosan-Treated RatsPubmed:29462796