ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42) Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Competition ELISA

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  • ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42) Packages (Simulation)
  • ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42) Packages (Simulation)
  • ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42) Results demonstration
  • CEA946Mi.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Amyloid Beta Peptide 1-42 (Ab1-42) and the recovery rates were calculated by comparing the measured value to the expected amount of Amyloid Beta Peptide 1-42 (Ab1-42) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-104 94
EDTA plasma(n=5) 97-105 102
heparin plasma(n=5) 85-101 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amyloid Beta Peptide 1-42 (Ab1-42) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amyloid Beta Peptide 1-42 (Ab1-42) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amyloid Beta Peptide 1-42 (Ab1-42) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 91-99% 90-99% 95-105%
EDTA plasma(n=5) 98-105% 79-104% 90-98% 88-101%
heparin plasma(n=5) 87-94% 79-97% 78-102% 83-93%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Amyloid Beta Peptide 1-42 (Ab1-42) and unlabeled Amyloid Beta Peptide 1-42 (Ab1-42) (Standards or samples) with the pre-coated antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Amyloid Beta Peptide 1-42 (Ab1-42) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Amyloid Beta Peptide 1-42 (Ab1-42) in the sample.

Citations

  • Blockade of Tau Hyperphosphorylation and Aβ1–42 Generation by the Aminotetrahydrofuran Derivative ANAVEX2-73, a Mixed Muscarinic and σ1 Receptor Agonist, in a Nontransgenic Mouse Model of Alzheimer’s DiseasePubmed: 23493042
  • The γ-secretase inhibitor 2-[(1R)-1-[(4-chlorophenyl)sulfonyl](2,5-difluorophenyl) amino]ethyl-5-fluorobenzenebutanoic acid (BMS-299897) alleviates Aβ1–42 seeding and short-term memory deficits in the Aβ25–35 mouse model of Alzheimer's diseasePubmed: 23123349
  • 慢病毒介导的ＣＢＳ基因过表达对原代皮质神经元Globesci:Source
  • 外源性硫化氢对高糖条件下原代神经元β淀粉样蛋白的影响J-Smu:Source
  • Effects of Aluminium on β-Amyloid (1–42) and Secretases (APP-Cleaving Enzymes) in Rat BrainPubmed:24792732
  • Amyloid ? lowering and cognition enhancing effects of ghrelin receptor analog [D-Lys (3)] GHRP-6 in rat model of obesityPubmed:25296496
  • Anesthetic Propofol Attenuates Apoptosis, Aβ Accumulation, and Inflammation Induced by Sevoflurane Through NF-κB Pathway in Human Neuroglioma CellsPubMed: 25809614
  • Autophagy dysfunction upregulates beta-amyloid peptides via enhancing the activity of γ-secretase complexPubMed: 26316755
  • Geniposide attenuates insulin-deficiency-induced acceleration of β-amyloidosis in an APP/PS2 transgenic model of Alzheimer&#039;s diseasePubMed: 25882166
  • The Potential Effect of Epigallocatechin-3-Gallate alone or in Combination with Vitamin E and Selenium on Alzheimer's Disease Induced by Aluminum in Ratsopen-access:
  • 芝麻素对高糖条件下PC12细胞Aβ和BACE-1表达的影响article:10299
  • Tong Luo Jiu Nao, a Chinese Medicine Formula, Reduces Inflammatory Stress in a Mouse Model of Alzheimer's DiseasePubmed:26993489
  • ОСОБЕННОСТИ ПРОЦЕССОВ ФОРМИРОВАНИЯ ПОКАЗАТЕЛЕЙ ФУНКЦИОНАЛЬНОГО СОСТОЯНИЯ ВЫСШЕЙ НЕРВНОЙ ДЕЯТЕЛЬНОСТИ И ХАРАКТЕРИСТИКИ ВНИМАНИЯ СТУДЕНТОВSMB-2016-03-062.pdf
  • 芝麻素对高糖条件下PC12 细胞Aß 和BACE 1 表达的影响QK:92621A
  • Geniposide attenuates the level of Aβ1–42 via enhancing leptin signaling in cellular and APP/PS1 transgenic micepubmed:28160136
  • Effects of dietary glucocerebrosides from sea cucumber on the brain sphingolipid profiles of mouse models of Alzheimer's disease.pubmed:28232989
  • Norovirus P particle-based active Aβ immunotherapy elicits sufficient immunogenicity and improves cognitive capacity in a mouse model of Alzheimer’s diseasearticles:srep41041.ris
  • Cerebrosides from Sea Cucumber Protect Against Oxidative Stress in SAMP8 Mice and PC12 Cellspubmed:28406733
  • Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat dietPubmed:29986309
  • Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female ratsPubmed:30028991
  • Sub-chronic celecoxib prevents soluble beta amyloid-induced depressive-like behaviour in ratsPubmed:29879605
  • Punicalagin ameliorates the elevation of plasma homocysteine, amyloid-β, TNF-α and apoptosis by advocating antioxidants and modulating apoptotic mediator proteins in brain modulating apoptotic mediator …Pubmed:29579708
  • Effects of Astaxanthin and Docosahexaenoic-Acid-Acylated Astaxanthin on Alzheimer's Disease in APP/PS1 Double-Transgenic MicePubmed:29695154
  • Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in ratsPubmed:29522778
  • CART peptide activates the Nrf2/HO-1 antioxidant pathway and protects hippocampal neurons in a rat model of Alzheimer's diseasePubmed:29777699
  • Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse …Pubmed:29731975
  • Ellagic acid ameliorates learning and memory impairment in APP/PS1 transgenic mice via inhibition of β‑amyloid production and tau hyperphosphorylationDoi: 10.3892/etm.2018.6860
  • 非编码RNA miR-137 调控tau 蛋白磷酸化和L 型钙离子通道CACNA1C 参与阿尔茨海默病的发生
  • (台灣農業研究67 (4): 355-364) 香菇柄水萃物改善糖尿病降低阿茲海默氏症罹病風險初探
  • The Efficacy of Sauropus androgynus Leaves Extract To Improve Cognitive Function in Wistar Rats Induced Alzheimer's
  • Effect of Aluminum-Maltolate on the Content of Aβ Protein and the Expression of ApoER2, VLDLRs, and LRP1 in PC12-ApoE4 CellsPubmed: 30649678
  • Imbalance of Microglial TLR4/TREM2 in LPS-Treated APP/PS1 Transgenic Mice: A Potential Link Between Alzheimer's Disease and Systemic InflammationPubmed: 30756214
  • Repetitive transcranial magnetic stimulation protects mice against 6-OHDA-induced Parkinson's disease symptoms by regulating brain amyloid β level
  • Obstructive sleep apnea may increase the risk of Alzheimer's diseasePubmed: 31487291
  • Nanoformulated ellagic acid ameliorates pentylenetetrazol-induced experimental epileptic seizures by modulating oxidative stress, inflammatory cytokines and …Pubmed: 31728888
  • Neurobiological determinants of depressive-like symptoms in rodents
  • Mahanimbine-induced neuroprotection via cholinergic system and attenuated amyloidogenesis as well as neuroinflammation in lipopolysaccharides-induced …
  • Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet.
  • Ex Vivo Investigation of Bexarotene and Nicotinamide Function as a Protect?ve Agent on Rat Synaptosomes Treated with A¦Â (1-42)33428094
  • Comparative study on the effects of phosphatidylserine rich in DHA and EPA on A¦Â-intervened Alzheimer's disease using cell models33876786
  • Protective effects of repetitive transcranial magnetic stimulation against 6-OHDA-induced Parkinson's symptoms in a mice model: the key role of miR-409-3p/PDHB ¡­34078216
  • Immunization with A¦Â3-10-KLH vaccine improves cognitive function and ameliorates mitochondrial dysfunction and reduces Alzheimer's disease-like pathology in Tg ¡­34044034
  • The effect of a 6-month Ketogenic Medium-Chain Triglyceride Supplement on Plasma Cardiometabolic and Inflammatory Markers in Mild Cognitive?¡­33906081
  • Potential Therapeutic Effects of New Ruthenium (III) Complex with Quercetin: Characterization, Structure, Gene Regulation, and Antitumor and Anti-Inflammatory Studies (RuIII/Q Novel Complex Is a Potent Immunoprotective Agent)
  • GPNMB mitigates Alzheimer's disease and enhances autophagy via suppressing the mTOR signal34695452
  • The influence of vinpocetine alone or in combination with Epigallocatechin-3-gallate, Coenzyme COQ10, Vitamin E and Selenium as a potential neuroprotective …34706318
  • Probiotics ameliorates LPS induced neuroinflammation injury on Aβ 1–42, APP, γ-β secretase and BDNF levels in maternal gut microbiota and fetal …Pubmed:35312928
  • New Thienopyridine Scaffold as An Antidementia Lead: In Silico Study and Biological Screening

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