ELISA Kit for Angiotensin 1-7 (Ang1-7) Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Competition ELISA

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  • ELISA Kit for Angiotensin 1-7 (Ang1-7) Packages (Simulation)
  • ELISA Kit for Angiotensin 1-7 (Ang1-7) Packages (Simulation)
  • ELISA Kit for Angiotensin 1-7 (Ang1-7) Results demonstration
  • CES085Mi.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Angiotensin 1-7 (Ang1-7) and the recovery rates were calculated by comparing the measured value to the expected amount of Angiotensin 1-7 (Ang1-7) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-98 91
EDTA plasma(n=5) 96-103 101
heparin plasma(n=5) 94-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin 1-7 (Ang1-7) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin 1-7 (Ang1-7) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin 1-7 (Ang1-7) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-104% 84-104% 86-96% 85-95%
EDTA plasma(n=5) 89-103% 99-105% 91-101% 79-101%
heparin plasma(n=5) 78-98% 90-97% 83-94% 84-92%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Angiotensin 1-7 (Ang1-7)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Angiotensin 1-7 (Ang1-7) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Angiotensin 1-7 (Ang1-7) and unlabeled Angiotensin 1-7 (Ang1-7) (Standards or samples) with the pre-coated antibody specific to Angiotensin 1-7 (Ang1-7). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin 1-7 (Ang1-7) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Angiotensin 1-7 (Ang1-7) in the sample.

Citations

  • Improvement of Plasma Biomarkers after Switching Stroke Patients from Other Angiotensin II Type I Receptor Blockers to OlmesartanPubMed: 25891757
  • Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Ratspubmed:28848201
  • Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expressionpubmed:29066981
  • The ACE2-Ang (1-7)-Mas receptor axis attenuates cardiac remodeling and fibrosis in post-myocardial infarction.pubmed:28656296
  • Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites.pubmed:28895159
  • Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive ratspubmed:28944898
  • A Study of the Correlation between Angiotensin (1-7) and the Histopathological Changes in the Penises of Experimentally Diabetic Rats2-1980272_83198.htm
  • Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin systemPubmed:29752040
  • Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axisPubmed:29969931
  • Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy SubjectsPubmed:29977313
  • The impact of ovulation inducing drugs on carcinogenesis
  • Changes of angiotensin II (1–8) and angiotensin (1–7) levels in early and late-onset preeclampsia
  • Therapeutic Delivery of Ang (1-7) Via Genetically Modified Probiotic: A Dosing Study.Pubmed: 31586210
  • Significance of the angiotensin I/angiotensin II/angiotensin‐(1‐7) axis in the pathogenesis of systemic sclerosisPubmed: 31746507
  • Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot studyPubmed: 32756181
  • Dikkat Eksikliği Hiperaktivite Bozukluğu Tanısı Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin Dönüştürücü Enzim (ACE), NADPH Oksidaz Enzim Serum …
  • Angiotensin-(1¨C7) attenuates collagen-induced arthritis via inhibiting oxidative stress in rats33398523
  • The prevalence and role of functional autoantibodies to angiotensin-converting-enzyme-2 in patients with systemic sclerosis33910447
  • Ang-(1-7) Protects Skeletal Muscle Function in Aged Mice
  • Angiotensin-(1-7) protects against sepsis-associated left ventricular dysfunction induced by lipopolysaccharide34298021
  • Ang-(1–7) protects skeletal muscle function in aged mice34548056
  • Resveratrol Confers Protection Against Ischemia/Reperfusion Injury by Increase of Angiotensin (1-7) Expression in a Rat Model of Myocardial Hypertrophy34232225

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