ELISA Kit for Anti-Myelin Basic Protein Antibody (Anti-MBP)
Myelin A1 protein; Myelin membrane encephalitogenic protein
- UOM
- FOB US$ 560.00 US$ 800.00 US$ 3,600.00 US$ 6,800.00 US$ 56,000.00
- Quantity
Overview
Properties
- Product No.AEA539Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antibody Detection.
Research use only - DownloadInstruction Manual
- CategoryInfection immunityNeuro scienceAutoimmunity
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Recovery
Matrices listed below were spiked with certain level of Anti-Myelin Basic Protein Antibody (Anti-MBP) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Myelin Basic Protein Antibody (Anti-MBP) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 80-101 | 91 |
EDTA plasma(n=5) | 81-99 | 94 |
heparin plasma(n=5) | 94-102 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Myelin Basic Protein Antibody (Anti-MBP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Myelin Basic Protein Antibody (Anti-MBP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Myelin Basic Protein Antibody (Anti-MBP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 99-105% | 88-98% | 87-97% | 98-105% |
EDTA plasma(n=5) | 99-105% | 91-102% | 80-101% | 87-101% |
heparin plasma(n=5) | 82-89% | 95-102% | 80-104% | 92-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Myelin Basic Protein Antibody (Anti-MBP) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Myelin Basic Protein Antibody (Anti-MBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Citations
- CD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
- Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat BrainPubmed: 23183024
- Myelin basic protein and ischemia modified albumin levels in acute ischemic stroke casesPubMed: 26648996
- Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus)Pubmed:27165445
- Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomyAA.2016.0008
- Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord.pubmed:27573615
- Glial markers and emotional memory in rats following acute cerebral radiofrequency exposures.pubmed:27696165
- Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomyAA.2016.0008
- Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosispubmed:28489854
- Acrolein-mediated neurotoxicity in growing Wistar male ratsPubmed:30033014
- Association and Risk of Canine Distemper with Respect to Age, Sex and Breed of Dogs Suffering from Demyelinating Neuropathies10.5455:ijlr.20170811080455
- Biosensors and nanobiosensors for rapid detection of autoimmune diseases: a reviewPubmed: 31760523
- The role of light desynchronosis in the development of stress-induced aging
- Association of eosinophil-mediated inflammatory biomarkers with the presence of the Schatzki ring34091432
- Searching for Noninvasive Predictors of the Diagnosis and Monitoring of Eosinophilic Esophagitis¡ªThe Importance of Biomarkers of the Inflammatory Reaction?¡
- Monitoring autoimmune diseases by bioelectrochemical detection of autoantibodies. Application to the determination of anti-myelin basic protein autoantibodies in …Pubmed:35202838