ELISA Kit for Anti-Pentraxin 3, Long Antibody (Anti-PTX3) Homo sapiens (Human) Antibody ELISA

TNFAIP5; TSG-14; Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit for Anti-Pentraxin 3, Long Antibody (Anti-PTX3) Packages (Simulation)
  • ELISA Kit for Anti-Pentraxin 3, Long Antibody (Anti-PTX3) Packages (Simulation)
  • ELISA Kit for Anti-Pentraxin 3, Long Antibody (Anti-PTX3) Results demonstration
  • AEK411Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Anti-Pentraxin 3, Long Antibody (Anti-PTX3) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Pentraxin 3, Long Antibody (Anti-PTX3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-102 97
EDTA plasma(n=5) 84-97 90
heparin plasma(n=5) 95-102 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Pentraxin 3, Long Antibody (Anti-PTX3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Pentraxin 3, Long Antibody (Anti-PTX3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Pentraxin 3, Long Antibody (Anti-PTX3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-98% 91-98% 98-105% 95-104%
EDTA plasma(n=5) 92-104% 78-90% 81-94% 81-88%
heparin plasma(n=5) 81-94% 87-95% 81-97% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Anti-Pentraxin 3, Long Antibody (Anti-PTX3)

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Pentraxin 3, Long Antibody (Anti-PTX3) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Pentraxin 3, Long Antibody (Anti-PTX3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis ModelPubMed: 22966213
  • Effect of Vagus Nerve Stimulation on Focal Transient Cerebral Ischemia and Reperfusion in Adult Male White New Zealand RabbitsSfu: Source
  • Hemodialysis-induced changes in the blood composition affect function of the endotheliumOnlinelibrary: hdi.12148
  • Hemodialysis‐induced changes in the blood composition affect function of the endotheliumPubmed:24612443
  • The effects of sunitinib on endometriosisPubmed:25093747
  • Exenatide improves ovarian and endometrial injury and preserves ovarian reserve in streptozocin induced diabetic ratsPubmed:25366063
  • Ovarian failure in diabetic rat model: Nuclear factor-kappaB, oxidative stress, and pentraxin-3Pubmed:25510691
  • Octreotide protects ovary against ischemia–reperfusion injury in rats: Evaluation of histological and biochemical parametersPubMed: 26223286
  • The long pentraxin-3 is a useful marker for diagnosis of ovarian torsion: An experimental rat modelPubMed: 26470829
  • Accumulation of α-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers5952149
  • Study on Association of Pentraxin 3 and Diabetic Nephropathy in a Rat ModelPubmed:29725602
  • The Relationship between Serum Pentraxin-3 Levels and Severity of Coronary Heart DiseasepaperID:86206
  • Serum levels and gene expression of pentraxin 3 are elevated in COPDPubmed: 30572222
  • Pentraxin 3 Detects Clinically Significant Fibrosis in Patients with Chronic Viral Hepatitis C
  • Jie Du Tong Ye San Prevents N-Nitrosomethylbenzylamine-Induced Esophageal Carcinogenesis via Inhibition of Inflammation and Proliferation
  • Study on Serum Pentraxin-3 Levels in Vasculitis with HypertensionPubmed: 31264920
  • The role of CRP and Pentraxin 3 in the prediction of systemic inflammatory response syndrome and death in acute pancreatitisPubmed: 31798002
  • Losartan ameliorates ovarian ischaemia/reperfusion injury in rats: an experimental studyPubmed: 31955629
  • Effects of Methylene Blue on Ovarian TorsionLDetorsion Injury in a Rat ModelPubmed: 32593749
  • Therapeutic effects of vitamin D on acetic acid-induced colitis in ratsPubmed: 32555936
  • Pentraxin 3 as a marker for cardiovascular disease risk in overweight and obese children33716459
  • Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­34035808
  • High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese …34684637
  • Protective effects of dichloroacetic acid on endometrial injury and ovarian reserve in an experimental rat model of diabetes mellitus34595798

Recommend products