ELISA Kit for Anti-S100 Calcium Binding Protein B Antibody (Anti-S100B) Homo sapiens (Human) Antibody ELISA

S100-B; NEF; S100Beta; S-100 protein subunit beta

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  • ELISA Kit for Anti-S100 Calcium Binding Protein B Antibody (Anti-S100B) Packages (Simulation)
  • ELISA Kit for Anti-S100 Calcium Binding Protein B Antibody (Anti-S100B) Packages (Simulation)
  • ELISA Kit for Anti-S100 Calcium Binding Protein B Antibody (Anti-S100B) Results demonstration
  • AEA567Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Anti-S100 Calcium Binding Protein B Antibody (Anti-S100B) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-S100 Calcium Binding Protein B Antibody (Anti-S100B) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-105 101
EDTA plasma(n=5) 85-98 90
heparin plasma(n=5) 94-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-S100 Calcium Binding Protein B Antibody (Anti-S100B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-S100 Calcium Binding Protein B Antibody (Anti-S100B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-S100 Calcium Binding Protein B Antibody (Anti-S100B) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-103% 92-104% 98-105% 85-98%
EDTA plasma(n=5) 87-101% 88-99% 97-105% 88-101%
heparin plasma(n=5) 82-105% 80-94% 86-99% 95-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Anti-S100 Calcium Binding Protein B Antibody (Anti-S100B)

Test principle

The microtiter plate provided in this kit has been pre-coated with S100B. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-S100B will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-S100B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Protective effects of naloxone in two-hit seizure modelPubMed: 19674055
  • The Anti-Inflammatory and Neuroprotective Effects of Ghrelin in Subarachnoid Hemorrhage-Induced Oxidative Brain Damage in RatsLiebert: 20091210
  • Sub-acute effects of diazinon on biochemical indices and specific biomarkers in rats: Protective effects of crocin and safranalPubMed: 20637253
  • Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladderWiley: source
  • A preliminary study of the effects of ulinastatin on early postoperative cognition function in patients undergoing abdominal surgeryPubmed: 23422155
  • Induction of receptor for advanced glycation end products by insufficient leptin action triggers pancreatic β-cell failure in type 2 diabetes.Pubmed: 23410183
  • The Experiment Research of Neuroprotection of Hypoperfusion Postconditioning on Cerebral IschemiaPubmed:25006960
  • The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesionsPubMed: 26184156
  • The Severity of Cecal Ligature and Puncture-Induced Sepsis Correlates with the Degree of Encephalopathy, but the Sepsis Does Not Lead to Acute Activation of Spleen Lymphocytes in MicePubMed: 26081155
  • Stężenie białka S100B w surowicy krwi u młodzieży z omdleniamiyadda:60e5630d0ac1
  • 右美托咪定对脑膜瘤切除术患者血清S100β 蛋白及神经元特异性烯醇化酶的影响article:9794
  • Development of an experimental rat model of hyperammonemic encephalopathy and evaluation of the effects of rifaximinPubmed:26980242
  • Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions.pubmed:27882497
  • Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acidpubmed:27490041
  • Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β …pubmed:29040978
  • Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanismspubmed:28061403
  • Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory …Pubmed:29672286
  • Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathwayPubmed:29510144
  • Protective Effects of Cornel Iridoid Glycoside in Rats After Traumatic Brain InjuryPubmed:29492766
  • C6 glioma-conditioned medium induces malignant transformation of mesenchymal stem cells: Possible role of S100B/RAGE pathwayPubmed:29050939
  • S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid …Pubmed:29801873
  • Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsyPubmed:29365285
  • S100B as a potential biomarker for concussion in male and female collegiate rugby athletes:
  • Serum S100B levels in patients with depression
  • Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway
  • Extracellular S100β Disrupts Bergman Glia Morphology and Synaptic Transmission in Cerebellar Purkinje CellsPubmed: 31013844
  • The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the …
  • Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion …Pubmed: 32214270
  • Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 …Pubmed: 32300366
  • Biomarkers of Neurological Outcome After Aneurysmal Subarachnoid Hemorrhage as Early Predictors at Discharge from an Intensive Care UnitPubmed: 32978732
  • Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and …Pubmed: 32432773
  • ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ …
  • Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD)33469081
  • Serum biomarkers and cerebral autoregulation as early warnings of delayed cerebral ischemia risk in patients after aneurysmal subarachnoid haemorrhage27885969
  • Increased GFAP concentrations in the cerebrospinal fluid of patients with unipolar depression34021122
  • Neuroprotective effect of 6-hydroxy-2, 2, 4-trimethyl-1, 2-dihydroquinoline mediated via regulation of antioxidant system and inhibition of inflammation and apoptosis in?¡­33964368
  • High S100B levels predict antidepressant response in patients with major depression even when considering inflammatory and metabolic markersPubmed:35176771
  • 1-benzoyl-6-hydroxy-2, 2, 4-trimethyl-1, 2-dihydroquinoline exerts a neuroprotective effect and normalises redox homeostasis in a rat model of cerebral ischemia …Pubmed:35201554
  • Neuroprotective effect of sodium alginate against chromium-induced brain damage in ratsPubmed:35421180
  • Sepsis Encephalopathy Is Partly Mediated by miR370-3p-Induced Mitochondrial Injury but Attenuated by BAM15 in Cecal Ligation and Puncture Sepsis Male MicePubmed:35628259
  • THE EVALUATION OF MEDULLA SPINALIS PERFUSION AT MODERATE HYPOTHERMIA WITH PATIENTS WHO UNDERGO ANTEGRADE SELECTIVE CEREBRAL …
  • EPIDEMIOLOGIA, DETERMINANTELE ȘI PREDICȚIA ACCIDENTULUI VASCULAR CEREBRAL ISCHEMIC LA COPII

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