ELISA Kit for Anti-Somatostatin Antibody (Anti-SST) Sus scrofa; Porcine (Pig) Antibody ELISA

GHIH; SRIF; SMST; GHRIH; GHRIF; Growth Hormone Releasing Inhibiting Hormone; Somatotropin Release-Inhibiting Factor; Somatostatin-14; Somatostatin-28

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  • ELISA Kit for Anti-Somatostatin Antibody (Anti-SST) Packages (Simulation)
  • ELISA Kit for Anti-Somatostatin Antibody (Anti-SST) Packages (Simulation)
  • ELISA Kit for Anti-Somatostatin Antibody (Anti-SST) Results demonstration
  • AEA592Po.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of Anti-Somatostatin Antibody (Anti-SST) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Somatostatin Antibody (Anti-SST) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-95 91
EDTA plasma(n=5) 87-94 91
heparin plasma(n=5) 92-102 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Somatostatin Antibody (Anti-SST) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Somatostatin Antibody (Anti-SST) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Somatostatin Antibody (Anti-SST) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-99% 85-101% 80-105% 98-105%
EDTA plasma(n=5) 87-104% 93-101% 84-91% 82-98%
heparin plasma(n=5) 79-104% 91-105% 79-103% 94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Anti-Somatostatin Antibody (Anti-SST)

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Somatostatin Antibody (Anti-SST) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Somatostatin Antibody (Anti-SST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Pancreatic Somatostatinoma Diagnosed Preoperatively: Report of a CasePubmed: 24413789
  • Circadian Modulation of Anxiety: A Role for Somatostatin in the AmygdalaPubmed: 24376834
  • Enhanced expression of neuropeptide S (NPS) receptor in eosinophils from severe asthmatics and subjects with total IgE above 100IU/mlPubmed:24239856
  • Inhibitory effect of somatostatin on insulin secretion is not mediated via the CNSPubmed:25630331
  • Oral curcumin has anti-arthritic efficacy through somatostatin generationPubMed: 25836921
  • Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor ratpubmed:27685769
  • Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation10.1007/s13765-017-0327-3
  • Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipationpubmed:29082242
  • Somatostatin Maintains Permeability and Integrity of Blood-Brain Barrier in β-Amyloid Induced ToxicityPubmed:29700775
  • Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile AcidsPubmed:29853946
  • Relationship between somatostatin and interleukin-6: A cross-sectional study in patients with acute pancreatitisPubmed: 30279074
  • Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in MicePubmed: 30404008
  • Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric UlcerPubmed: 30479041

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