ELISA Kit for Arachidonic Acid (AA) Pan-species (General) Competition ELISA


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  • ELISA Kit for Arachidonic Acid (AA) Packages (Simulation)
  • ELISA Kit for Arachidonic Acid (AA) Packages (Simulation)
  • ELISA Kit for Arachidonic Acid (AA) Results demonstration
  • CEB098Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered


Matrices listed below were spiked with certain level of Arachidonic Acid (AA) and the recovery rates were calculated by comparing the measured value to the expected amount of Arachidonic Acid (AA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-101 97
EDTA plasma(n=5) 95-105 99
heparin plasma(n=5) 95-103 101


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arachidonic Acid (AA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arachidonic Acid (AA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Arachidonic Acid (AA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-95% 94-103% 90-98% 83-94%
EDTA plasma(n=5) 98-105% 79-96% 92-99% 82-90%
heparin plasma(n=5) 80-103% 82-96% 97-105% 79-98%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1 Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Reagent Diluent 1×300µL Stop Solution 1×6mL
TMB Substrate 1×9mL Instruction manual 1
Wash Buffer (30 × concentrate) 1×20mL

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Arachidonic Acid (AA)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Arachidonic Acid (AA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Arachidonic Acid (AA) and unlabeled Arachidonic Acid (AA) (Standards or samples) with the pre-coated antibody specific to Arachidonic Acid (AA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Arachidonic Acid (AA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Arachidonic Acid (AA) in the sample.


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