ELISA Kit for Brain Derived Neurotrophic Factor (BDNF) Mus musculus (Mouse) Sandwich ELISA

Neurotrophin; Abrineurin

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  • ELISA Kit for Brain Derived Neurotrophic Factor (BDNF) Packages (Simulation)
  • ELISA Kit for Brain Derived Neurotrophic Factor (BDNF) Packages (Simulation)
  • ELISA Kit for Brain Derived Neurotrophic Factor (BDNF) Results demonstration
  • SEA011Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Brain Derived Neurotrophic Factor (BDNF) and the recovery rates were calculated by comparing the measured value to the expected amount of Brain Derived Neurotrophic Factor (BDNF) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-95 91
EDTA plasma(n=5) 96-104 101
heparin plasma(n=5) 80-93 85

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Brain Derived Neurotrophic Factor (BDNF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-90% 98-105% 96-103% 96-105%
EDTA plasma(n=5) 97-105% 90-97% 78-104% 98-105%
heparin plasma(n=5) 93-102% 91-101% 81-88% 80-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Brain Derived Neurotrophic Factor (BDNF)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Derived Neurotrophic Factor (BDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Brain Derived Neurotrophic Factor (BDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat BrainsPubMed: 21436126
  • Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cordScienceDirect: S0022510X12001219
  • Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative DamagePsrcentre: 212059
  • Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone miceScienceDirect: S0304394010015788
  • Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord.NCBI: PMC3925622
  • The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factorPubmed:25446810
  • Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord.Pubmed:26559686
  • Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young ratspmc:PMC4812310
  • Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cellsPubmed:27323064
  • Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study articles:Vol14Iss3
  • Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons.pubmed:27914953
  • PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice.pubmed:27940378
  • Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalitiespubmed:28081639
  • An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …articles:journal-of-alzheimers-disease
  • Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic …bdi.12449
  • Associations of blood parameters with age, feed efficiency and sampling routine in young beef bullsscience:article
  • Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitispubmed:28412394
  • A new comorbidity model and the common pathological mechanisms of migraine and epilepsypubmed:28559979
  • Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal.pubmed:29042206
  • An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signalingPubmed:29627530
  • Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse modelPubmed:29335016
  • N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotypePubmed:29203442
  • Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemiaPubmed:30012511
  • Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model.Pubmed:29890692
  • Delta-9-Tetrahydrocannabinol (∆-THC) Induce Neurogenesis and Improve Cognitive Performances of Male Sprague Dawley RatsPubmed:28933048
  • Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in ratsPubmed:29522778
  • Long term Westernized diet leads to region-specific changes in brain signaling mechanismsPubmed:29655945
  • Changes in exercise capacity and serum BDNF following long-term sprint interval training in well-trained cyclistsPubmed: 30286300
  • Establishment and evaluation of a novel mouse model of peri/postmenopausal depressionDoi: 10.1016/j.heliyon.2019.e01195
  • Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling
  • Effect of voluntary running on expression of myokines in brains of rats with depressionPubmed: 30834799
  • Clinical usefulness of brain-derived neurotrophic factor and visinin-like protein-1 in early diagnostic tests for acute strokePubmed: 30826210
  • Monoaminergic system is implicated in the antidepressant-like effect of hyperoside and protocatechuic acid isolated from Impatiens glandulifera Royle in micePubmed: 31077758
  • Physical Exercise Increased Brain-Derived Neurotrophic Factor in Elderly Population with DepressionPubmed: 30994834
  • Regular Exercise Enhances Cognitive Function and Intracephalic GLUT Expression in Alzheimer's Disease Model MicePubmed: 31561359
  • Cognitive Decline of Rats with Chronic Fluorosis Is Associated with Alterations in Hippocampal Calpain SignalingPubmed: 31797207
  • Chronic harmine treatment has a delayed effect on mobility in control and socially defeated ratsPubmed: 32088835
  • Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicityPubmed: 32010279
  • Association of neuronal repair biomarkers with delirium among survivors of critical illnessPubmed: 31896448
  • CSF levels of a set of neurotrophic factors (brain-derived neurotrophic factor, nerve growth factor) and neuropeptides (neuropeptide Y, galanin) in epileptic childrenPubmed: 32327377
  • Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and …Pubmed: 32918393
  • Impaired LTD-like motor cortical plasticity in female patients with major depression disorderPubmed: 32791084
  • Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's diseasePubmed: 32777662
  • Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in micePubmed: 32522471
  • Comparison of serum brain-derived neurotrophic factor (BDNF) in dogs with and without separation anxiety
  • Nicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial …
  • Short-but no long-term effect of social stress in rodent depressive-like behavior is not affected by chronic treatment with Harmine
  • Blood Plasma Trophic Growth Factors Predict the Outcome in Patients with Acute Ischemic Stroke
  • Overektomize dişi sıçanlarda beyin oksidatif stres, bdnf ve inflamatuar cevap düzeyleri ile nörodavranışsal değişiklikler üzerine anjiyotensin II İnhibisyonunun etkisi
  • Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­33389158
  • Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways33589896
  • Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567 …34239303
  • Tropomyosin receptor kinase B-mediated signaling in integration of neuropathic pain and obesity in diabetic polyneuropathy34586159
  • Moderate-intensity continuous training and high-intensity interval training improve cognition, and BDNF levels of middle-aged overweight men34762211
  • Lithium augmentation of ketamine increases insulin signaling and antidepressant-like active stress coping in a rodent model of treatment-resistant depression34824208
  • Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons34712429
  • Endogenous Deficiency of Brain-Derived Neurotrophic Factor Induces the Downregulation of Tryptophan Hydroxylase-2 Expression in Raphe Nuclei of Rapid …34384732
  • CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway34296452
  • Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader …Pubmed:35098494
  • Gender influence on clinical manifestations, depressive symptoms and brain-derived neurotrophic factor (BDNF) serum levels in patients affected by …Pubmed:35344113
  • Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patientsPubmed:35508633
  • Co-Delivery of Docosahexaenoic Acid and Brain-Derived Neurotropic Factor from Electrospun Aligned Core–Shell Fibrous Membranes in Treatment of Spinal Cord …Pubmed:35214053
  • Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377
  • Effect of herbal formulation Sharbat Ahmad Shahi on serum BDNF level in mild to moderate cases of depressive disorder
  • Medicinal effects of Saffron on obese animals by reducing Castellis risk index to protect from cardio-pathology and psychological distress

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