ELISA Kit for C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) Mus musculus (Mouse) Sandwich ELISA

CTRP9; C1QTNF9A; AQL1; Complement C1q and tumor necrosis factor-related protein 9A

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  • ELISA Kit for C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) Packages (Simulation)
  • ELISA Kit for C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) Packages (Simulation)
  • ELISA Kit for C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) Results demonstration
  • SER877Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) and the recovery rates were calculated by comparing the measured value to the expected amount of C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-102 98
EDTA plasma(n=5) 87-96 91
heparin plasma(n=5) 81-98 87

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 98-105% 97-105% 97-105%
EDTA plasma(n=5) 92-101% 78-105% 85-103% 79-99%
heparin plasma(n=5) 84-101% 95-103% 93-101% 94-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Association of serum C1q/TNF-Related Protein-9 (CTRP9) concentration with visceral adiposity and metabolic syndrome in humans.Pubmed: 24357853
  • Association of Serum C1q/TNF-Related Protein-9 Concentration With Arterial Stiffness in Subjects With Type 2 DiabetesPubmed:25105737
  • C1q/TNF-Related Protein-9 (CTRP9) Levels Are Associated With Obesity and Decrease Following Weight Loss SurgeryPubmed:26982010
  • Circulating C1q complement/TNF-related protein (CTRP) 1, CTRP9, CTRP12 and CTRP13 concentrations in Type 2 diabetes mellitus: In vivo regulation by glucose.pubmed:28207876
  • Plasma C1q/TNF-Related Protein-9 Levels Are Associated with Atherosclerosis in Patients withType 2 Diabetes without Renal Dysfunction.pubmed:28070523
  • Circulating CTRP9 levels are increased in patients with newly diagnosed type 2 diabetes and correlated with insulin resistancepubmed:28743061
  • Association of circulating CTRP9 with soluble adhesion molecules and inflammatory markers in patients with type 2 diabetes mellitus and coronary artery …Pubmed:29381773
  • Effect of a single bout of resistance exercise on zinc-α2-glycoproteinPubmed: 31686532
  • Association of serum CTRP9 levels with cardiac autonomic neuropathy in patients with type 2 diabetes mellitus33417302
  • Association Between Serum C1q Tumor Necrosis Factor-Related Protein 9 and the Clinical Characteristics and Prognosis of Ischemic Stroke34727346

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