ELISA Kit for Carbonic Anhydrase VI (CA6) Mus musculus (Mouse) Sandwich ELISA

CA-VI; CAVI; GUSTIN; Carbonate dehydratase VI; Salivary carbonic anhydrase; Secreted carbonic anhydrase

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  • ELISA Kit for Carbonic Anhydrase VI (CA6) Packages (Simulation)
  • ELISA Kit for Carbonic Anhydrase VI (CA6) Packages (Simulation)
  • ELISA Kit for Carbonic Anhydrase VI (CA6) Results demonstration
  • SED073Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Carbonic Anhydrase VI (CA6) and the recovery rates were calculated by comparing the measured value to the expected amount of Carbonic Anhydrase VI (CA6) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-97 93
EDTA plasma(n=5) 90-98 94
heparin plasma(n=5) 86-97 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase VI (CA6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase VI (CA6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Carbonic Anhydrase VI (CA6) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-95% 78-99% 91-99% 87-94%
EDTA plasma(n=5) 84-99% 90-104% 85-92% 81-91%
heparin plasma(n=5) 93-101% 92-101% 80-104% 89-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Carbonic Anhydrase VI (CA6)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase VI (CA6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase VI (CA6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase VI (CA6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase VI (CA6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Salivary markers of taste sensitivity to oleic acid: a combined proteomics and metabolomics approachSpringer: Source
  • SALIVARY CARBONIC ANHYDRASE VI, ZINC SULFATE TASTE ACUITY AND FREQUENCY OF ILLNESS: A PILOT STUDY Com:Source
  • Associations between food consumption patterns and saliva composition: Specificities of eating difficulties children.pubmed:28185876
  • Differences in the density of fungiform papillae and composition of saliva in patients with taste disorders compared to healthy controlspubmed:28981826
  • Children with a Higher Activity of Carbonic Anhydrase VI in Saliva Are More Likely to Develop Dental Caries.pubmed:28633135
  • Obese Subjects With Specific Gustatory Papillae Microbiota and Salivary Cues Display an Impairment to Sense LipidsPubmed:29713004
  • Acceptance of added fat to first complementary feeding purees: An exploration of fat type, feeding history and saliva compositionPubmed: 30172680
  • Activity of Carbonic Anhydrase VI is Higher in Dental Biofilm of Children with CariesPubmed: 31151296
  • Polymorphism in the CAVI gene, salivary properties and dental cariesPubmed: 32013665

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