ELISA Kit for Copeptin (CPP) Homo sapiens (Human) Competition ELISA

C-Terminal Provasopressin; Vasopressin-Neurophysin 2-Copeptin

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  • ELISA Kit for Copeptin (CPP) Packages (Simulation)
  • ELISA Kit for Copeptin (CPP) Packages (Simulation)
  • ELISA Kit for Copeptin (CPP) Results demonstration
  • CEA365Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Copeptin (CPP) and the recovery rates were calculated by comparing the measured value to the expected amount of Copeptin (CPP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-102 97
EDTA plasma(n=5) 87-101 95
heparin plasma(n=5) 94-105 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Copeptin (CPP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Copeptin (CPP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Copeptin (CPP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-95% 89-103% 92-101% 99-105%
EDTA plasma(n=5) 81-89% 89-104% 85-98% 82-101%
heparin plasma(n=5) 90-104% 78-103% 99-105% 87-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for Copeptin (CPP)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Copeptin (CPP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Copeptin (CPP) and unlabeled Copeptin (CPP) (Standards or samples) with the pre-coated antibody specific to Copeptin (CPP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Copeptin (CPP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Copeptin (CPP) in the sample.

Citations

  • Copeptin: May it be a novel biomarker for insulin theraphy in diabetes? An animal study.Ejmanager: Source
  • Serum copeptin in children exposed to maltreatmentPubmed:27278269
  • Methods for Diagnosis and Treatment of Concussion or Brain Injury2016:0103140
  • Role of enteroendocrine L‐cells in arginine vasopressin‐mediated inhibition of colonic anion secretionPubmed:27037495
  • Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic …files:ijcem0031358.pdf
  • Orthostatic Challenge Shifts the Hemostatic System of Patients Recovered from Stroke toward Hypercoagulability.pubmed:28223937
  • N-Terminal Prohormone of Brain Natriuretic Peptide but not C-Terminal Pre-Pro Vasopressin (Copeptin) Level is Associated with the Response to Antihypertensive …pubmed:29190613
  • Early Prediction of Preeclampsiajournals:297397
  • Evaluation of Urinary Aquaporin 2 and Plasma Copeptin as Biomarkers of Effectiveness of Desmopressin Acetate for the Treatment of Monosymptomatic Nocturnal Enuresis.pubmed:28457803
  • Experimental Heat Stress Nephropathy and Liver Injury are Improved by AllopurinolPubmed:29667911
  • Angiotensin AT Receptors Expressed in Vasopressin-Producing Cells of the Supraoptic Nucleus Contribute to the Osmotic Control of VasopressinPubmed:29364700
  • Rehydration with fructose worsens dehydration-induced renal damagePubmed:30005632
  • Copeptin level in the early prediction of cardiorenal syndrome in ratsPubmed:30112047
  • 和肽素水平对大鼠心肾综合征的早期预测价值filename:ZBLS201802013
  • Abstract P311: Static Urine Osmolality With Elevated First Trimester Urine Copeptin in Human Preeclampsia
  • Assessment of serum levels of copeptin and corticotropin-releasing factor in children with monosymptomatic and nonmonosymptomatic nocturnal enuresis
  • Cognitive performance is associated with cerebral oxygenation and peripheral oxygen saturation, but not plasma catecholamines, during graded normobaric hypoxia
  • Dialysate copeptin and peritoneal transport in incident peritoneal dialysis patientsPubmed: 31187425
  • The contribution of collecting duct NOS1 to the concentrating mechanisms in male and female micePubmed: 31241990
  • The increase of core temperature affected the progression of kidney injury by repeated heat stress exposurePubmed: 31390229
  • Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletionPubmed: 31863073
  • Copeptin is independently associated with vascular calcification in chronic kidney disease stage 5Pubmed: 32033584
  • Micro RNA 146a gene variant/TNF-α/IL-6/IL-1 β; A cross-link axis inbetween oxidative stress, endothelial dysfunction and neuro-inflammation in acute ischemic stroke …Pubmed: 31738890
  • Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with ObesityPubmed: 33027789
  • Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker StudyPubmed: 32549327
  • Relation of Copeptin with Diabetic and Renal Function Markers Among Patients with Diabetes Mellitus Progressing Towards Diabetic NephropathyPubmed: 32505416
  • 重组人脑利钠肽治疗慢性肺心病急性加重期的临床效果及对5-HT 浓度的影响
  • Validity of different copeptin assays in the differential diagnosis of the polyuria-polydipsia syndrome33980941
  • Are the Circulating Levels of Copeptin and Fibronectin Dysregulated in Preeclamptic South African Black Women?
  • Copeptin levels are more elevated and associated with cardiovascular events in the unity of chronic ischemic heart and chronic kidney diseases
  • High serum copeptin may be a marker of an increased carotid intima-media thickness in asymptomatic patients with type 1 diabetes.
  • The Speed of Ingestion of a Sugary Beverage Has an Effect on the Acute Metabolic Response to Fructose
  • Dietary K+ acts as a genuine diureticPubmed:34984847
  • Spectrally multiplexed assay using gap enhanced nanoparticle for detection of a myocardial infarction biomarker panelPubmed:35190126
  • Arcuate NPY is involved in salt‐induced hypertension via modulation of paraventricular vasopressin and brain‐derived neurotrophic factorPubmed:35312067
  • Copeptin Concentrations in Plasma of Healthy Neonates in Relation to Water–Electrolyte Homeostasis in the Early Adaptation PeriodPubmed:35327814

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