ELISA Kit for D-Amino Acid Oxidase (DAO) 
DAMOX
- UOM
- FOB US$ 490.00 US$ 700.00 US$ 3,150.00 US$ 5,950.00 US$ 49,000.00
- Quantity
Overview
Properties
- Product No.SEJ298Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
Research use only - DownloadInstruction Manual
- CategoryEnzyme & Kinase
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of recombinant D-Amino Acid Oxidase (DAO) and the recovery rates were calculated by comparing the measured value to the expected amount of D-Amino Acid Oxidase (DAO) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 89-96 | 93 |
| EDTA plasma(n=5) | 84-101 | 94 |
| heparin plasma(n=5) | 87-104 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Amino Acid Oxidase (DAO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of D-Amino Acid Oxidase (DAO) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-92% | 87-96% | 98-105% | 95-103% |
| EDTA plasma(n=5) | 88-99% | 79-93% | 95-104% | 98-105% |
| heparin plasma(n=5) | 90-97% | 89-97% | 97-105% | 84-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-Amino Acid Oxidase (DAO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D-Amino Acid Oxidase (DAO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D-Amino Acid Oxidase (DAO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D-Amino Acid Oxidase (DAO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Giveaways
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Single-component Reagents of Assay Kit
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Lysis Buffer Specific for ELISA / CLIA
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Quality Control of Kit
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ELISA Kit Customized Service
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Disease Model Customized Service
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TGFB1 Activation Reagent
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Streptavidin
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Streptavidin-Agarose Beads
Citations
- Differential regulation of D-amino acid oxidase (DAO) mRNA and protein expression in human post-mortem brainDam: Jcr:3107E495-Ef68-4692-85Be-D43D9F3F84Ff
- Age span expression of schizophrenia susceptibility genes D-amino acid oxidase (DAO) and DAO activator (DAOA) in human post-mortem braindam:jcr:c3394762-a692-4fbd-8a37-7da14f429996
- Expression of D-Amino Acid Oxidase (DAO/DAAO) and D-Amino Acid Oxidase Activator (DAOA/G72) during Development and Aging in the Human Post-mortem Brain.pubmed:28428746
- Noninvasive biomarkers of gut barrier function identify two subtypes of patients suffering from diarrhoea predominant-IBS: a case-control studyPubmed: 30400824
- Gut microbiota dysbiosis worsens the severity of acute pancreatitis in patients and micePubmed: 30519748
- Effect of a chamomile extract in protecting against radiation‐induced intestinal mucositisPubmed: 30632234
- Role of Aquaporin-3 in Intestinal Injury Induced by SepsisPubmed: 31582652
- Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory responsePubmed: 32361189
- Effect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injury33514869
- The Relationship between Low Serum Vitamin D Levels and Altered Intestinal Barrier Function in Patients with IBS Diarrhoea Undergoing a Long-Term Low-FODMAP33801020
- The ¦Á2AR/Caveolin©\1/p38MAPK/NF©\¦ÊB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia©\reperfusion34114328
- Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity34022802
- Psychological and Gastrointestinal Symptoms of Patients with Irritable Bowel Syndrome Undergoing a Low-FODMAP Diet: The Role of the Intestinal Barrier34371976
- Blood D-amino acid oxidase (DAO) levels increased with cognitive decline among people with mild cognitive impairment (MCI): a two-year prospective studyPubmed:35430632