ELISA Kit for D-Dimer (D2D) Rattus norvegicus (Rat) Competition ELISA

D 2 Dimer

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  • ELISA Kit for D-Dimer (D2D) Packages (Simulation)
  • ELISA Kit for D-Dimer (D2D) Packages (Simulation)
  • ELISA Kit for D-Dimer (D2D) Results demonstration
  • CEA506Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant D-Dimer (D2D) and the recovery rates were calculated by comparing the measured value to the expected amount of D-Dimer (D2D) in samples.

Matrix Recovery range (%) Average(%)
sodium citrate plasma(n=5) 84-99 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Dimer (D2D) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Dimer (D2D) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of D-Dimer (D2D) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 85-93% 79-92% 93-102% 82-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

ELISA Kit for D-Dimer (D2D)

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to D-Dimer (D2D) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled D-Dimer (D2D) and unlabeled D-Dimer (D2D) (Standards or samples) with the pre-coated antibody specific to D-Dimer (D2D). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of D-Dimer (D2D) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of D-Dimer (D2D) in the sample.

Citations

  • Determinants of the thrombogenic potential of multiwalled carbon nanotubesScienceDirect: S0142961211004819
  • Exogenous Activated Protein C Inhibits the Progression of Diabetic NephropathyPubmed: 22236035
  • Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in MicePlosOne: Source
  • Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central IndiaPubmed: 23992124
  • Assessment of coagulopathy, endothelial injury, and inflammation after traumatic brain injury and hemorrhage in a porcine modelLww:Source
  • Protein S exacerbates alcoholic hepatitis by stimulating liver natural killer T cellsPubmed:25399514
  • Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous CrisisPubmed:25448261
  • Impact of β-Adrenoceptor Blockade on Systemic Inflammation and Coagulation Disturbances in Rats with Acute Traumatic CoagulopathyPubmed:25676919
  • Intraperitoneal administration of activated protein C prevents postsurgical adhesion band formation.PubMed: 25575539
  • Procoagulant and Fibrinolytic Activity after Polytrauma in RatPubMed: 26632604
  • Upregulation of canonical transient receptor potential channel in the pulmonary arterial smooth muscle of a chronic thromboembolic pulmonary hypertension rat modelPubMed: 26155749
  • Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptorsPubMed: 25546502
  • KLF2 and KLF4 control endothelial identity and vascular integrity.pubmed:28239661
  • Activated protein C drives the hyperfibrinolysis of acute traumatic coagulopathypubmed:27841821
  • Activated TAFI Promotes the Development of Chronic Thromboembolic Pulmonary Hypertension: A Possible Novel Therapeutic Targetpubmed:28289017
  • Inhibition of Cell Apoptosis and Amelioration of Pulmonary Fibrosis by Thrombomodulinpubmed:28739343
  • Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin.pubmed:28923781
  • Lactadherin Promotes Microvesicle Clearance to Prevent Coagulopathy and Improves Survival of Severe TBI Micepubmed:29162596
  • Guided longer pulses from a diagnostic ultrasound and intraclot microbubble enhanced catheter-directed thrombolysis in vivo.pubmed:28417266
  • Experimental Evaluation of a New Tissue Factor-Based Topical Hemostat (TT-173) for Treatment of Hepatic BleedingPubmed: 30380352
  • The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in RodentsPubmed: 31533230
  • Complement factor D is linked to platelet activation in human and rodent sepsis34396466
  • NLRP3 Inflammasome contributes to endotoxin-induced coagulationPubmed:35421682
  • Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosisPubmed:35126715

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