ELISA Kit for Filaggrin (FLG) Homo sapiens (Human) Sandwich ELISA

Profilaggrin

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  • ELISA Kit for Filaggrin (FLG) Packages (Simulation)
  • ELISA Kit for Filaggrin (FLG) Packages (Simulation)
  • ELISA Kit for Filaggrin (FLG) Results demonstration
  • SEJ103Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Filaggrin (FLG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Filaggrin (FLG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Filaggrin (FLG)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filaggrin (FLG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filaggrin (FLG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filaggrin (FLG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filaggrin (FLG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Filaggrin R501x and 2282del4 gene mutations and its tissue levels in patients with chronic plaque psoriasisLWW: 01000
  • Neuromodulatory and Anti-Inflammatory Ingredient for Sensitive Skin: In Vitro Assessment Pubmed:24931812
  • Protective Effects of Triphala on Dermal Fibroblasts and Human KeratinocytesPubmed:26731545
  • In vitro anti-inflammatory and skin protective properties of extract comprising ceramides from de-oiled rice brandoi:10.1111
  • Imiquimod-induced psoriasis-like inflammation in differentiated Human keratinocytes: Its evaluation using curcumin.pubmed:28736282
  • A novel, topical, nonsteroidal, TRPV1 antagonist, PAC-14028 cream improves skin barrier function and exerts anti-inflammatory action through modulating epidermal …Pubmed:29752146
  • In vitro anti-inflammatory and skin protective properties of Virgin coconut oil10.1016:j.jtcme.2017.06.012
  • Markers of atopic dermatitis, allergic rhinitis and bronchial asthma in pediatric patients: correlation with filaggrin, eosinophil major basic protein and …
  • Filaggrin, major basic protein and leukotriene B4: Biomarkers for adult patients of bronchial asthma, atopic dermatitis and allergic rhinitisPubmed: 30560019
  • Epidermal and Dermal Hallmarks of Photoaging are Prevented by Treatment with Night Serum Containing Melatonin, Bakuchiol, and Ascorbyl …
  • 糖化に起因する表皮機能低下に対するワイルドタイムエキスの防御効果
  • Filaggrin‐gene mutation has minimal effect on the disease severity in the lesions of atopic dermatitis34322929

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