ELISA Kit for Follicle Stimulating Hormone (FSH) Rhesus monkey (Simian) Sandwich ELISA

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  • ELISA Kit for Follicle Stimulating Hormone (FSH) Packages (Simulation)
  • ELISA Kit for Follicle Stimulating Hormone (FSH) Packages (Simulation)
  • ELISA Kit for Follicle Stimulating Hormone (FSH) Results demonstration
  • SEA830Si.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Follicle Stimulating Hormone (FSH) and the recovery rates were calculated by comparing the measured value to the expected amount of Follicle Stimulating Hormone (FSH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-105 99
EDTA plasma(n=5) 82-96 90
heparin plasma(n=5) 82-98 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Follicle Stimulating Hormone (FSH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-103% 91-99% 78-104% 91-105%
EDTA plasma(n=5) 80-101% 94-103% 88-95% 85-93%
heparin plasma(n=5) 86-99% 98-105% 92-105% 95-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Follicle Stimulating Hormone (FSH)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Follicle Stimulating Hormone (FSH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Follicle Stimulating Hormone (FSH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Follicle Stimulating Hormone (FSH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Follicle Stimulating Hormone (FSH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β,7β,17β-triol, a synthetic anti-inflammatory androstene Ijcem: IJCEM1102004
  • Reproductive Toxicity of Tobacco Shisha Smoking on Semen Parameters and Hormones Levels among Adult Egyptian MenSciAlert: source
  • Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic ratsExcli: Source
  • Central effects of camphor on GnRH and sexual hormones in male ratPubmed: 24551777
  • Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in RatsPubmed: 24051982
  • The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar ratsInformahealthcare: Source
  • Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female ratsIjppsjournal: Source
  • Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized ratsPubmed:24316378
  • FoxO1 Is a Negative Regulator of FSHβ Gene Expression in Basal and GnRH-Stimulated Conditions in FemalePubmed:24437485
  • Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testisPubmed:24576220
  • Endocrine disrupting effects of dichlorodiphenyltrichloroethane analogues on gonadotropin hormones in pituitary gonadotrope cellsPubmed:24814263
  • Perfluorooctane sulfonate effects on the reproductive axis in adult male ratsPubmed:25171141
  • Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertilityPubmed:25505846
  • Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapyTurkishjournalofurology:Source
  • Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in RatsPubMed: 26629940
  • Protective effects of Eugenia jambolana extract versus N‐acetyl cysteine against cisplatin‐induced damage in rat testisPubMed: 24576220
  • The effect of R-(-)-deprenyl administration on reproductive parameters of rat malesPubMed: 25725114
  • Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar ratsPubMed: 26123866
  • Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female ratsPubMed: 26476359
  • Sex-specific prenatal stress effects on the rat reproductive axis and adrenal gland structurePubmed:27026714
  • Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat OffspringPubmed:26962114
  • Dose- and time-dependent effects of Garcinia kola seed extract on sexual behaviour and reproductiveparameters in male Wistar rats.Pubmed:26123866
  • Molecular characterization of insulin resistance and glycolytic metabolism in the rat uteruspubmed:27461373
  • Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.pubmed:28336389
  • Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum.pubmed:27838061
  • Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats.pubmed:28000943
  • Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring.pubmed:26962114
  • Impact of uteroplacental insufficiency on postnatal rat male gonad.pubmed:27885054
  • New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency.pubmed:28232269
  • Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axispubmed:28731686
  • Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activitypubmed:28958147
  • Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cellspubmed:29149324
  • Vitamin D deficiency impairs testicular development and spermatogenesis in micepubmed:28655646
  • Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis.pubmed:28578315
  • High sensitivity of testicular function to titanium nanoparticlespubmed:28496042
  • The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic micepubmed:29058779
  • Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathwaypubmed:29020999
  • Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspringpubmed:28687593
  • FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control studySuppl_2:728.1.abstract
  • The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicityPubmed:30021657
  • Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island populationPubmed:29525625
  • Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in MicePubmed:29403355
  • Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle lossPubmed:29351580
  • DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathwayPubmed:29321035
  • Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) …Pubmed:29627845
  • Palladium nanoparticle effects on endocrine reproductive system of female ratsPubmed:29405765
  • A Pex13 knockout in germ cells induces a spermatogenic arrestvolltexte:2018/8921/
  • Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neuronsDoi: 10.1016/j.biopha.2018.08.135
  • Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in RatsPubmed: 30569668
  • Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndromePubmed: 29535146
  • Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary
  • Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve
  • G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide …Pubmed: 31034039
  • The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.
  • Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in …Pubmed: 31311554
  • Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male ratsPubmed: 31353623
  • Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinasePubmed: 31722718
  • Sleep deprivation decreases the reproductive capacity by affecting the arrival of morulas in the uterusPubmed: 31815354
  • Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signalingPubmed: 31809938
  • Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary MorphologyPubmed: 32120970
  • Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 …Pubmed: 33131727
  • Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance …Pubmed: 33109277
  • MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistancePubmed: 32757043
  • Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic …Pubmed: 32698821
  • Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal ModelPubmed: 32517356
  • N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in ratsPubmed: 32447106
  • Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female micePubmed: 32407858
  • Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat StressPubmed: 32685488
  • Evaluation for adverse effects of InP/ZnS quantum dots on the in vitro cultured oocytes of mice
  • Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS
  • Hemin Ameliorated Letrozole-Induced Polycystic Ovary Syndrome: A Forward Step for Better Management
  • The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice
  • Drug-Herb Interaction Between Selective Estrogen Receptor Modulators and Estrogenic Herbal Medicine Er-Xian Decoction in Bone in Vivo and in Vitro
  • Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis33422655
  • The Effects of Zinc Methionine on Reproductive and Thyroid Hormones in Rats with Polycystic Ovarian Syndrome33457340
  • Diabetes Type 1 Negatively Influences Leydig Cell Function in Rats, Which is Partially Reversible By Insulin Treatment33507237
  • BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose33508368
  • Water extract of Er-xian decoction selectively exerts estrogenic activities and interacts with SERMs in estrogen-sensitive tissues33823166
  • Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion33965371
  • Lipoamino Acid-Modified GnRH Analogs with Receptor-Mediated Antiproliferative Activity in Prostate and Ovarian Cancer Cells
  • Regulation of Uterine Function During Estrous Cycle, Anestrus Phase and Pregnancy by Steroids in Red Deer (Cervus elaphus L.)
  • Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice
  • Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency
  • Human and animal fertility studies in cystinosis reveal signs for obstructive azoospermia, a disruptive blood‐testis barrier and a subtherapeutic effect of cysteamine in …34494673
  • MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 134637688
  • Cadmium attenuates testosterone synthesis by promoting ferroptosis and blocking autophagosome-lysosome fusion34610361
  • Endothelial dysfunction after androgen deprivation therapy and the possible underlying mechanisms34570375
  • Polyamine metabolism links gut microbiota and testicular dysfunction34758869
  • Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice34730391
  • Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in …34733331
  • METTL3-mediated mRNA N6-methyladenosine is required for oocyte and follicle development in mice34689175
  • Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with …Pubmed:35063921
  • Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome MicePubmed:35057459
  • TrkB agonist antibody ameliorates fertility deficits in aged and cyclophosphamide-induced premature ovarian failure model micePubmed:35177657
  • Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expressionPubmed:35276145
  • Constitutional delay of growth and puberty in female mice is induced by circadian rhythm disruption in uteroPubmed:35679725
  • G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and …
  • Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency
  • A promising animal model for Polycystic Ovary Syndrome: Borderline Hypertensive Rats

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