ELISA Kit for Follistatin Like Protein 1 (FSTL1) Rattus norvegicus (Rat) Sandwich ELISA

FRP; FSL1; Follistatin-related protein 1

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  • ELISA Kit for Follistatin Like Protein 1 (FSTL1) Packages (Simulation)
  • ELISA Kit for Follistatin Like Protein 1 (FSTL1) Packages (Simulation)
  • ELISA Kit for Follistatin Like Protein 1 (FSTL1) Results demonstration
  • SEJ085Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Follistatin Like Protein 1 (FSTL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Follistatin Like Protein 1 (FSTL1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 82-92 88
EDTA plasma(n=5) 90-101 93
heparin plasma(n=5) 86-104 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follistatin Like Protein 1 (FSTL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follistatin Like Protein 1 (FSTL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Follistatin Like Protein 1 (FSTL1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-101% 82-89% 85-102% 78-101%
EDTA plasma(n=5) 83-103% 95-104% 85-104% 95-104%
heparin plasma(n=5) 91-101% 98-105% 94-101% 93-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Follistatin Like Protein 1 (FSTL1)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Follistatin Like Protein 1 (FSTL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Follistatin Like Protein 1 (FSTL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Follistatin Like Protein 1 (FSTL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Follistatin Like Protein 1 (FSTL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Serum S100A8/A9, but not follistatin-like protein 1 and interleukin 18, may be a useful biomarker of disease activity in adult-onset Still's disease.PubMed: 22660800
  • Regulation of follistatin-like protein 1 expression and secretion in primary human skeletal muscle cellsPubmed: 23419164
  • Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferationPubmed:25118038
  • Growth Differentiation Factor-15 (GDF-15) Levels Are Associated with Cardiac and Renal Injury in Patients Undergoing Coronary Artery Bypass Grafting with Cardiopulmonary BypassPubmed:25171167
  • Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistancePubMed: 26199111
  • Association of Circulating Follistatin-Like 1 Levels with Inflammatory and Oxidative Stress Markers in Healthy Menpmc:PMC4856269
  • Follistatin like 1 Regulates Hypertrophy in Heart Failure with Preserved Ejection Fraction.pubmed:27430031
  • Follistatin like-1 aggravates silica-induced mouse lung injuryPMC5428474
  • Plasma follistatin-like protein 1 is correlated with disease severity in patients with acute pulmonary embolism and predicts short-term mortalityISSN:1936-2625
  • High circulating follistatin-like protein 1 as a biomarker of a metabolically unhealthy statePubmed: 30745500
  • Associations among circulating levels of follistatin-like 1, clinical parameters, and cardiovascular events in patients undergoing elective percutaneous …Pubmed: 31034503
  • Improved early detection of ovarian cancer using longitudinal multimarker modelsPubmed: 31937926
  • Targeting Follistatin like 1 ameliorates liver fibrosis induced by carbon tetrachloride through TGF-β1-miR29a in micePubmed: 32933544
  • Effects of acute endurance exercise on follistatin-like 1 and apelin in the circulation and metabolic organs in ratsPubmed: 32412800
  • Prognostic impact of transcardiac gradient of follistatin-like 1 reflecting hemodynamics in patients with dilated cardiomyopathy34334268
  • Association between Plasma Follistatin-like Protein 1 Levels and the Presence and Severity of Coronary Artery Disease34853217

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