ELISA Kit for Glucose 6 Phosphate Isomerase (GPI) Rattus norvegicus (Rat) Sandwich ELISA

AMF; NLK; PHI; SA36; Phosphoglucose Isomerase; Autocrine motility factor; Neuroleukin; Phosphohexose isomerase; Sperm antigen 36

Add to Cart Distributors
Overview
Properties
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • ELISA Kit for Glucose 6 Phosphate Isomerase (GPI) Packages (Simulation)
  • ELISA Kit for Glucose 6 Phosphate Isomerase (GPI) Packages (Simulation)
  • ELISA Kit for Glucose 6 Phosphate Isomerase (GPI) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Glucose 6 Phosphate Isomerase (GPI) and the recovery rates were calculated by comparing the measured value to the expected amount of Glucose 6 Phosphate Isomerase (GPI) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-97 92
EDTA plasma(n=5) 84-98 93
heparin plasma(n=5) 98-105 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucose 6 Phosphate Isomerase (GPI) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucose 6 Phosphate Isomerase (GPI) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glucose 6 Phosphate Isomerase (GPI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-103% 95-103% 79-90% 86-97%
EDTA plasma(n=5) 79-104% 80-90% 90-99% 94-105%
heparin plasma(n=5) 98-105% 93-104% 85-93% 83-91%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Glucose 6 Phosphate Isomerase (GPI)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucose 6 Phosphate Isomerase (GPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucose 6 Phosphate Isomerase (GPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucose 6 Phosphate Isomerase (GPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucose 6 Phosphate Isomerase (GPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factorPubMed: 23027359
  • Bruton's tyrosine kinase deficiency inhibits autoimmune arthritis but fails to block immune complex‐mediated inflammatory arthritisPubmed:26945549
  • Bruton's Tyrosine Kinase Deficiency Inhibits Autoimmune Arthritis in Mice but Fails to Block Immune Complex–Mediated Inflammatory Arthritispubmed:26945549
  • The GPI transamidase complex subunit PbGPI16 of Plasmodium berghei is important for inducing experimental cerebral malariaPubmed:29784863
  • Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motilityPubmed: 31394311
  • The Asthma-associated PER1-like domain-containing protein 1 (PERLD1) Haplotype Influences Soluble Glycosylphosphatidylinositol Anchor Protein (sGPI …Pubmed: 31959860
  • T–B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated ProteinPubmed: 33199538

Recommend products