ELISA Kit for Growth Differentiation Factor 11 (GDF11) Mus musculus (Mouse) Sandwich ELISA

BMP11; Bone morphogenetic protein 11

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  • ELISA Kit for Growth Differentiation Factor 11 (GDF11) Packages (Simulation)
  • ELISA Kit for Growth Differentiation Factor 11 (GDF11) Packages (Simulation)
  • ELISA Kit for Growth Differentiation Factor 11 (GDF11) Results demonstration
  • SEC113Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Growth Differentiation Factor 11 (GDF11) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Differentiation Factor 11 (GDF11) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-96 92
EDTA plasma(n=5) 98-105 102
heparin plasma(n=5) 95-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 11 (GDF11) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 11 (GDF11) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Differentiation Factor 11 (GDF11) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-95% 79-92% 85-99% 89-96%
EDTA plasma(n=5) 78-93% 79-98% 84-92% 84-102%
heparin plasma(n=5) 93-101% 86-98% 82-101% 79-92%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Growth Differentiation Factor 11 (GDF11)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 11 (GDF11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 11 (GDF11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Differentiation Factor 11 (GDF11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Differentiation Factor 11 (GDF11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Citations

  • Splenocytes derived from young WT mice prevent AD progression in APPswe/PSENldE9 transgenic micePubMed: 26317549
  • TGFβ Superfamily Members Mediate Androgen Deprivation Therapy-Induced Obese Frailty in Male Micepubmed:27611336
  • GDF11 is increased in patients with myelodysplastic syndromefiles:ijcep0024948.pdf
  • GDF11 does not improve the palmitate induced insulin resistance in C2C12.pubmed:28485800
  • РОЛЬ ДИФФЕРЕНЦИРОВОЧНОГО ФАКТОРА РОСТА 11 (GDF11) В РЕГУЛЯЦИИ ЛИПИДНОГО ОБМЕНА И КАРДИОГЕМОДИНАМИЧЕСКИХ ФУНКЦИЙ …Cyberleninka:Source
  • GDF11 is increased in patients with aplastic anemiaPubmed: 30727851
  • GDF11 prevents the formation of thoracic aortic dissection in mice: Promotion of contractile transition of aortic SMCs33764670
  • The relationship between transforming growth factor β superfamily members (GDF11 and BMP4) and lumbar spine bone mineral density in postmenopausal Chinese …34417839
  • Low-Intensity Exercise Routine for a Long Period of Time Prevents Osteosarcopenic Obesity in Sedentary Old Female Rats, by Decreasing Inflammation and …34336096

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